英语翻译1,and C.McSweeney2,1State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing,China,2CSIRO Livestock Industries,Queensland,Australia.The purpose of the present study was to inves

英语翻译1,and C.McSweeney2,1State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing,China,2CSIRO Livestock Industries,Queensland,Australia.The purpose of the present study was to inves
英语翻译
1,and C.McSweeney2,
1State Key Laboratory of Animal Nutrition,
Institute of Animal Science,Chinese Academy of Agricultural Sciences,
Beijing,China,
2CSIRO Livestock Industries,Queensland,Australia.
The purpose of the present study was to investigate the probiotic effect
of Bacillus subtilis (natto) on the rumen bacterial diversity of weaning
calves.Eight calves were randomly assigned into two groups,a control
group fed a starter diet,and a treatment group supplemented with Bacil-
lus subtilis (natto),which was mixed with the diet at a concentration
of 5×106 CFU per gram of the average daily feed intake.Calves were
slaughtered eight weeks after weaning.Rumen contents were taken
from the perforated rumen immediately.DNA was extracted using
the Beater-Bead method and used for 16S rDNA library construction.
Results showed that feeding Bacillus subtilis (natto) decreased the
concentration of volatile fatty acids in the rumen of the treatment group
compared with the control group (67.9 vs.73.5 mmol/L).The vast major-
ity of the two 16S rDNA clone libraries were represented by sequences
related to the Bacteroidetes and Firmicutes.Members of Bacteroidetes
accounted for about 39% and 25% of the total clones in control and
treatment group,respectively,while Firmicutes increased from 46% in
control to 57% in the treatment group.Clones affiliated to Ruminococ-
cus and Prevotella accounted for 5% and 32% in control while 10%
and 17% in the treatment animals respectively.These differences were
confirmed by real-time PCR quantification using genus-specific prim-
ers.The numbers of R.albus (log10 7.7 per mL) and R.flavefaciens
(log10 8.1 per mL) were higher in the rumens of the treatment group
than in that of control group (log10 7.3 per mL and log10 7.7 per mL,
respectively).These results indicated that supplementation of Bacillus
subtilis (natto) on weaning calves could promote the establishment of
the rumen microbial community,especially the growth of fibrolytic

英语翻译1,and C.McSweeney2,1State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing,China,2CSIRO Livestock Industries,Queensland,Australia.The purpose of the present study was to inves
1,和C.麦斯威尼2,1State动物营养重点实验室,动物科学研究所,中国科学院,北京农业科学院,中国,2CSIRO畜牧业,澳大利亚昆士兰州.本研究的目的是探讨枯草杆菌（纳豆菌）对断奶犊牛瘤胃细菌多样性的益生菌作用.八犊牛,随机分成两组,一组喂以首发的饮食,治疗组与Bacil - LU的枯草补充（纳豆菌）,这是与饮食混合在5 × 106个菌落形成单位,每克的平均浓度每日采食量.犊牛断奶后屠宰八周.瘤胃内容取自立即穿孔瘤胃.提取DNA,用打浆机珠法和16S rDNA的图书馆建设.结果表明,饲养枯草芽孢杆菌（纳豆菌）降低了治疗组瘤胃挥发性脂肪酸的浓度较对照组（67.9 vs.73.5 mmol / L的）.广袤的少将两个16S rDNA克隆库,景军出席了有关Bacteroidetes和厚壁菌序列.对Bacteroidetes成员占约39％和25年的控制和治疗组,分别占总克隆％,而厚壁菌比例从46％控制在治疗组为57％.隶属于Ruminococ - CuS和普里沃无性系占5％和32％,而对照的10％和17％,治疗动物的分别.这些差异证实了实时PCR使用属特异性引物定量分析.r的黄鳝号码（每毫升7.7 LOG10的）和R.flavefaciens（每毫升8.1 LOG10的）在治疗组的瘤胃高于对照组（每毫升LOG10的7.3和7.7 log10的每毫升,分别） .这些结果表明,枯草芽孢杆菌对断奶犊牛（纳豆菌）补充可以促进瘤胃微生物群落的建立,特别是纤维分解增长
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