英语翻译The real-time PCR reactions were carried out using EXPRESS SYBER® GreenER™ qPCR SuperMix Universal (Invitrogen) in triplicates for each cDNA sample in the presence of mouse gene-specific primers forDicer(Forward:5′-AGTGTAGCCT

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英语翻译Thereal-timePCRreactionswerecarriedoutusingEXPRESSSYBER®GreenER™qPCRSuperMixUniversal

英语翻译The real-time PCR reactions were carried out using EXPRESS SYBER® GreenER™ qPCR SuperMix Universal (Invitrogen) in triplicates for each cDNA sample in the presence of mouse gene-specific primers forDicer(Forward:5′-AGTGTAGCCT
英语翻译
The real-time PCR reactions were carried out using EXPRESS SYBER® GreenER™ qPCR SuperMix Universal (Invitrogen) in triplicates for each cDNA sample in the presence of mouse gene-specific primers forDicer(Forward:5′-AGTGTAGCCTTAGCCATTTGC-3′;Reverse:5′-CTGGTGGCTTGAGGACAAGAC-3′).
机器翻的自己走.

英语翻译The real-time PCR reactions were carried out using EXPRESS SYBER® GreenER™ qPCR SuperMix Universal (Invitrogen) in triplicates for each cDNA sample in the presence of mouse gene-specific primers forDicer(Forward:5′-AGTGTAGCCT
用EXPRESS SYBER®GreenER™qPCR SuperMix染色剂（Invitrogen,英杰生命技术有限公司出品）以一式三份针对每个cDNA（互补脱氧核糖核酸）样本,在存在针对Dicer酶（正向：5'-AGTGTAGCCTTAGCCATTTGC-3';反向：5'-CTGGTGGCTTGAGGACAAGAC-3'）的小鼠基因特异性引物的情况下进行了实时PCR（聚合酶链锁反应）反应.
说明：EXPRESS SYBER®GreenER™qPCR SuperMix无法翻译,是Invitrogen公司生产的染色剂,这是牌号、注册商标.
Forward:5′-AGTGTAGCCTTAGCCATTTGC-3′;Reverse:5′-CTGGTGGCTTGAGGACAAGAC-3′
也无法翻译.
Dicer酶：是RNase III家族中特异识别双链RNA的一员,它能以一种ATP依赖的方式逐步切割由外源导入或者由转基因,病毒感染等各种方式引入的双链RNA,切割将RNA降解为19-21bp的双链RNAs(siRNAs),每个片段的3’端都有2个碱基突出.

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