英语翻译Change in spectral features of the copper complexes on interaction with CT-DNA in 50 nM Tris-HCl/18 mM NaCl buffer（pH=7.2）shift of ligand-band or charge-transfer bands.All the present complexes exhibit significant hypochromism (33.85-

英语翻译Change in spectral features of the copper complexes on interaction with CT-DNA in 50 nM Tris-HCl/18 mM NaCl buffer（pH=7.2）shift of ligand-band or charge-transfer bands.All the present complexes exhibit significant hypochromism (33.85-
英语翻译
Change in spectral features of the copper complexes on interaction with CT-DNA in 50 nM Tris-HCl/18 mM NaCl buffer（pH=7.2）shift of ligand-band or charge-transfer bands.All the present complexes exhibit significant hypochromism (33.85-44.26%) on the incremental addition of DNA with varying red shifts.Furthermore,as the extent of hypochromism is commonly consistent with the strength of intercalative interaction ,this indicates that complex 3 exhibits better DNA binding affinity compared with complexes 1,4 and 5.As a means for further clarifying the binding of complexes fluorescence spectral measurements were carried out.The addition of complex to the DNA bound EB solutions caused obvious reduction in emission intensities,indicating that complex competitively bound to DNA with EB.The extent of reduction of the emission intensity gives a measure of the binding propensity of the complex to DNA.According to the classical Stern-Volmer equation29 ...;I 0 and I are the fluorescence intensities in the absence and presence of the quencher,respectively.K is a linear Stern-Volmer quenching constant.[Q]is the concentration of the quencher.The quenching plots illustrate that the quenching of EB bound to DNA by complexes are in agreement with the linear Stern-Volmer equation ,which also indicate the complexes,K is given by the ratio of the slope to intercept .According to the equation ,where the complex concentration is the value for a 50% reduction of the fluorescence intensity of EB the K (app) values are calculated to be 4.67*10^5,9.48*10^5,4.30*10^5 and 3.90*10^5 M^-1 for complexes 1,3,4 and 5,less than the binding constant of the classical intercalators and metallointercalators(10^7 M^-1),17 which suggest that the interactions of the complexes with DNA are all moderate intercalative modes.Complex 3 is the best binding agent due to its highest planarity.30
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英语翻译Change in spectral features of the copper complexes on interaction with CT-DNA in 50 nM Tris-HCl/18 mM NaCl buffer（pH=7.2）shift of ligand-band or charge-transfer bands.All the present complexes exhibit significant hypochromism (33.85-
仅供参考
改变在光谱特征的铜配合物在交互与ct dna在50 nM三hcl / 18毫米生理盐水缓冲(pH = 7.2)转变的配位体带或电荷转移.
目前所有的配合物具有明显的减色性(33.85 - -44.26%)的增加与不同的DNA红光偏移.此外,随着程度通常符合减色性力量的变形交互,这表明复杂3展品更好的DNA亲和力与复合物1、4和5.作为一种手段,进一步阐明了荧光光谱测量绑定复合物进行了.添加复杂的DNA绑定EB解决方案造成明显的降低排放强度,表明复杂的竞争性必然的DNA与EB.减少的程度的排放强度给出了一个方法绑定倾向的复杂的DNA.根据古典Stern-Volmer equation29…… ;我0和我是荧光强度在缺乏和存在的饮料,分别.凯西是一个线性Stern-Volmer淬火常数.(问)的浓度冷却器.淬火图表明,淬火的EB绑定到DNA复合物在赞同线性Stern-Volmer方程,这也表明该复合物,K是给定的比率边坡的拦截.根据方程,复杂的浓度是价值的减少50%的荧光强度EB(app)的K值计算是4.67 * 10 ^ 5,9.48 * 10 ^ 5,4.30 * 10 ^ 5和3.90 * 10 ^ 5 M ^ 1复合物1、3、4和5,不到的结合常数的古典intercalators和metallointercalators(10 ^ 7 M ^ 1),17显示的交互配合物与DNA都是温和的intercalative模式.复杂的3是最好的绑定剂由于其最高平面化为30
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