英语翻译Factorial design and response surface techniques were used to optimizethe culture medium for the production of inulinase by Kluyveromycesmarxianus.Sucrose was used as the carbon source instead of inulin.Initially,a fractional factorial de

英语翻译Factorial design and response surface techniques were used to optimizethe culture medium for the production of inulinase by Kluyveromycesmarxianus.Sucrose was used as the carbon source instead of inulin.Initially,a fractional factorial de
英语翻译
Factorial design and response surface techniques were used to optimize
the culture medium for the production of inulinase by Kluyveromyces
marxianus.Sucrose was used as the carbon source instead of inulin.Initially,
a fractional factorial design (25–1) was used in order to determine the most
relevant variables for enzyme production.Five parameters were studied
(sucrose,peptone,yeast extract,pH,and K2HPO4),and all were shown to be
significant.Sucrose concentration and pH had negative effects on inulinase
production,whereas peptone,yeast extract,and K2HPO4 had positive ones.
The pH was shown to be the most significant variable and should be preferentially
maintained at 3.5.According to the results from the first factorial
design,sucrose,peptone,and yeast extract concentrations were selected to
be utilized in a full factorial design.The optimum conditions for a higher
enzymatic activity were then determined:14 g/L of sucrose,10 g/L of yeast
extract,20 g/L of peptone,1 g/L of K2HPO4.The enzymatic activity in the
culture conditions was 127 U/mL,about six times higher than before the
optimization.

英语翻译Factorial design and response surface techniques were used to optimizethe culture medium for the production of inulinase by Kluyveromycesmarxianus.Sucrose was used as the carbon source instead of inulin.Initially,a fractional factorial de
析因设计和响应面方法进行了优化培养基为菊粉酶生产kluyveromycesmarxianus.蔗糖作为碳源,而菊糖.最初,一个部分因子设计（25–1）是用来确定mostrelevant变量酶的生产.五个参数进行了研究（蔗糖,酵母提取物,蛋白胨,酸度,和K2HPO 4）,以及所有被证明besignificant.蔗糖浓度和PH值的负面影响了inulinaseproduction,而蛋白胨,酵母提取物,和K2HPO 4有正面.值被证明是最重要的变量和应preferentiallymaintained 3.5.根据结果从第一factorialdesign,蔗糖,蛋白胨和酵母提取物的浓度,被选定为在利用全因子设计.最佳条件为higherenzymatic活动被确定：14克／升的蔗糖,酵母膏10克／升,20克／升的蛋白胨,1克／升的磷酸氢二钾.酶活性在培养条件是127 /毫升,高约六倍,比优化前.