英语翻译Objective To observe the in vitro growth inhibitory and apopto sis-inducing effects of paclitaxel on the human osteosarcoma cell line U2- OS.Methods U-2 OS cells were treated with various concentrations of paclitaxel.Proliferation was det

英语翻译Objective To observe the in vitro growth inhibitory and apopto sis-inducing effects of paclitaxel on the human osteosarcoma cell line U2- OS.Methods U-2 OS cells were treated with various concentrations of paclitaxel.Proliferation was det
英语翻译
Objective To observe the in vitro growth inhibitory and apopto sis-inducing effects of paclitaxel on the human osteosarcoma cell line U2- OS.
Methods U-2 OS cells were treated with various concentrations of paclitaxel.Proliferation was determined by cell count in a neubauer cytometer chamber1.Viability was assessed by trypan blue dye exclusion.
Paclitaxel-induced morphologic alterations were visualized using light and transmission electron microscopy.The extent of paclitaxel-induced apoptosis was determined by flow cytometry and immunohistochemical
detection (TdT2-ediated dUTP nickend labeling technique ,TUNEL) .
Results The cells treated with paclitaxel initially showed G2/ M arrest ,which was followed by apoptosis.The characteristic apoptotic changes ,including nuclear disintegration and chromatin agglomeration ,were
displayed.Large amounts of micronuclei cells appeared ,something not observed in those cells treated with
cisplatin and adriamycin for contrast.Also ,extensive DNA-cleavage was detected using TUNEL.
Conclusion This study demonstrates that paclitaxel has an apoptotic-inducing effect on osteo sarcoma cells through the initiation of G2/ M arrest and inhibiting mitosis in both a time-dependent and dose-dependent manner.

英语翻译Objective To observe the in vitro growth inhibitory and apopto sis-inducing effects of paclitaxel on the human osteosarcoma cell line U2- OS.Methods U-2 OS cells were treated with various concentrations of paclitaxel.Proliferation was det
目的：观察紫杉醇对人体内骨肉瘤细胞系U2-OS生长的抑制和诱导凋亡作用.
方法：用不同浓度的紫杉醇处理骨肉瘤细胞系U2-OS.增殖能力由血细胞计数器计算得到的细胞数目决定.生存能力以对台盼蓝染料的排斥性来评定.紫杉醇诱导产生的形态学上的变化用光透射显微镜来观察,诱导程度由流式细胞仪和免疫组织化学检测（TdT介导的dUTP切口末端标记技术－TUNEL）.
结果：用紫杉醇处理过的细胞在细胞凋亡后表现为G2/M期阻滞.凋亡的特征改变了,表现为细胞核解体和染色质凝聚,还出现了许多微核细胞,这是用顺式铂氨和亚德里亚霉素处理过的作为对照的细胞没有出现的现象.同时,用TUNEL技术还发现了普遍的DNA裂解.
结论：这项研究表明了紫杉醇通过时间和剂量依赖性的对G2/M期阻滞和有丝分裂的抑制对骨肉瘤细胞起到诱导凋亡的作用.
正好,我这儿也有一篇一模一样的文章.