英语翻译Method Development for a Qualitative Analysis of LFP from Beef Heart MitochondriaThe chromatographic separation was carried out with an injection of chloroform LFP extracts into the system with¬out prior solvent evaporation,for chlor

英语翻译Method Development for a Qualitative Analysis of LFP from Beef Heart MitochondriaThe chromatographic separation was carried out with an injection of chloroform LFP extracts into the system with¬out prior solvent evaporation,for chlor
英语翻译
Method Development for a Qualitative Analysis of LFP from Beef Heart Mitochondria
The chromatographic separation was carried out with an injection of chloroform LFP extracts into the system with¬out prior solvent evaporation,for chloroform by no means worsened the separation.
When choosing the optimal mobile phase,we started with different mixtures of aceto- nitrile,methanol and water that proved to be good choices as running phases in our previous experiments.
However,LFP mixtures from mitochondria were not resolved well when using various combinations of these solvents.
After that,we did experiments with several different mobile phases composed of either methanol and water or aceto- nitrile and water,where the percentage of the non-polar solvent ranged from 30 to 80%.
The mobile phases con¬sisting of methanol and water were not efficient enough to clearly separate all fractions.
Using a mobile phase with acetonitrile as a non-polar solvent resulted in a better sep¬aration of different peaks,where the actual volume fraction of acetonitrile depended on the analyzed samples.
We first analyzed LFP obtained after in vitro incubation of mito¬chondria with MDA at 25 °C.
Comparing the chromato¬grams obtained with different volume fractions of acetonitrile used,it was found that mobile phase made of 60% acetonitrile,v/v,gave the best separation of individual peaks.

英语翻译Method Development for a Qualitative Analysis of LFP from Beef Heart MitochondriaThe chromatographic separation was carried out with an injection of chloroform LFP extracts into the system with¬out prior solvent evaporation,for chlor
定性分析方法发展的牛肉粒线体.从心LFP
进行了色谱分离与氯仿提取物注射进系统LFP¬溶剂挥发出来之前,氯仿绝不会恶化分离.
优化流动相的选择时,我们开始用不同的混合物aceto -作业、甲醇和水,提供了良好的运行阶段选择在我们先前的实验.
然而,由线粒体LFP混合物没有解决好当使用的各种组合这些溶剂.
在那之后,我们做实验具有不同的流动相的组成和水或甲醇aceto -作业和水,哪里的无极的比例范围从80%到的溶剂.
移动阶段的欺诈sisting¬甲醇和水不足够有效率清楚分开的所有分数.
使用移动阶段,一个无极溶剂乙腈导致更好的体感诱发电位(sep)¬你们不同的山峰,在实际的体积乙腈依靠分析样本.
我们首先分析了LFP体外孵育,mito¬chondria在25°C和丙二醛含量.
比较了不同chromato¬克的体积与乙腈使用,结果表明流动相由60%的乙腈,v / v,给出了最佳分离的个体的山峰上.