pBR有多克隆位点么?谢谢~pUC series is similar to pBR40% of the DNA left, including tetracycline resistance has been deletedCloning sites are clustered together into one area called the multiple cloning site (MCS)这个讲的蛮不清楚的.
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pBR有多克隆位点么?谢谢~pUC series is similar to pBR40% of the DNA left, including tetracycline resistance has been deletedCloning sites are clustered together into one area called the multiple cloning site (MCS)这个讲的蛮不清楚的.
pBR有多克隆位点么?谢谢~
pUC series is similar to pBR
40% of the DNA left, including tetracycline resistance has been deleted
Cloning sites are clustered together into one area called the multiple cloning site (MCS)
这个讲的蛮不清楚的.到底是相似点还是不同点?
pBR有多克隆位点么?谢谢~pUC series is similar to pBR40% of the DNA left, including tetracycline resistance has been deletedCloning sites are clustered together into one area called the multiple cloning site (MCS)这个讲的蛮不清楚的.
pBR322没有MCS,但其具有多个单一的限制性内切酶位点,其中7种内切酶的识别位点在四环素抗性基因内部,2种识别位点在这个基因的启动区内,所以9个限制酶切位点插入外源片段可以导致tetr 基因的失活；
另外有3种限制酶在氨苄青霉素抗性基因有单一的识别位点,插入外源片段可导致Ampr失活.
pUC质粒载体的优点 ：
第一, 具有较小的分子量和更高的拷贝数; 仅保留了pBR322的氨苄青霉素抗性基因和复制起点；
第二,具有来自大肠杆菌lac操纵子的lacZ’基因,可用于蓝白斑筛选；
第三,具有人工构建的多克隆位点MCS区段．方便具有不同粘性末端的外源片断的插入.