英语翻译Enzymic activitiesCell-free extracts were prepared from cells grown in thepresence of 2 mM sodium cyanide.Cells were harvested at the latest stage of the exponential growth and resuspendedin 50 mM Tris/HCl buffer (pH 8.5).Cells were broke
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英语翻译Enzymic activitiesCell-free extracts were prepared from cells grown in thepresence of 2 mM sodium cyanide.Cells were harvested at the latest stage of the exponential growth and resuspendedin 50 mM Tris/HCl buffer (pH 8.5).Cells were broke
英语翻译
Enzymic activities
Cell-free extracts were prepared from cells grown in the
presence of 2 mM sodium cyanide.Cells were harvested at the latest stage of the exponential growth and resuspended
in 50 mM Tris/HCl buffer (pH 8.5).Cells were broken by
cavitation,three pulses of 5 s at 90W.After centrifugation at
19 000 g,supernatants were collected and used as a source
of enzymes.Cyanase activity was assayed by a modified
method described previously [15].β-Cyanoalanine nitrilase
activity was assayed at 35◦C in the presence of 10 mM
β-cyanoalanine,50 mM Tris/HCl buffer (pH 8.5) and the
appropriate amount of enzyme.Samples were collected at
various times and ammonium production was determined as
described above.
英语翻译Enzymic activitiesCell-free extracts were prepared from cells grown in thepresence of 2 mM sodium cyanide.Cells were harvested at the latest stage of the exponential growth and resuspendedin 50 mM Tris/HCl buffer (pH 8.5).Cells were broke
从在
2mM的氰化钠存在下培养的细胞中酶活性的影响,制备无细胞提取物.上面的指数增长的最新阶段,收获细胞,重新悬浮
在50mM的Tris / HCl缓冲液（pH 8.5）中.细胞被打断
气蚀,3个5秒的脉冲90W.
19 000克离心后,收集上清液作为
酶源使用.cyanase活性测定由
前面描述的[15] 的变形方法.β-氰丙氨酸腈水解酶的
活性测定在35度下的10mM
β-氰丙氨酸的50mM Tris / HCl缓冲液（pH 8.5）中和
相应的酶量存在.样本收集在
不同的时间被确定为
上述铵生产.