酶切与PCR之间有什么联系又有什么区别
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酶切与PCR之间有什么联系又有什么区别
酶切与PCR之间有什么联系
又有什么区别
酶切与PCR之间有什么联系又有什么区别
1.When you put restriction sites at the end of PCR products,you have to consider giving some extra nucleotides at the end,because some of the enzymes do not cut so well if there are too few nucleotides at the end.
2.PCR product does not have modification,such as methylation.It is not supercoiled DNA either.Therefore,when you like to restrict PCR products,you may need a little longer time for the reaction,comparing to restrict plasmid DNA.
3.The oligos,and others,such as DNA polymerase,left in your PCR mix may interfere with the restriction,therefore,you may want to clean the PCR product before you are doing restriction.
Good luck!
首先说区别，酶切是把连续的碱基序列（DNA或者RNA）切断，PCR是把单个的核苷酸单体连接成链状DNA。
硬说他们有联系的话，只能这么理解，基因工程过程中，构建质粒载体的时候，我们先PCR扩增目的基因，然后经过酶切之后，得到合适的末端，这样就可以把目的基因跟载体连接起来了。...
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首先说区别，酶切是把连续的碱基序列（DNA或者RNA）切断，PCR是把单个的核苷酸单体连接成链状DNA。
硬说他们有联系的话，只能这么理解，基因工程过程中，构建质粒载体的时候，我们先PCR扩增目的基因，然后经过酶切之后，得到合适的末端，这样就可以把目的基因跟载体连接起来了。
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