repression domain SRDX---cres-t原理是什么呢?研究转录因子功能,转录因子缺失表达时,由于转录因子同源家族基因功能补偿,使得表型中很难辨别,cres-t能解决这个问题,那么具体原理是什么呢?
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repression domain SRDX---cres-t原理是什么呢?研究转录因子功能,转录因子缺失表达时,由于转录因子同源家族基因功能补偿,使得表型中很难辨别,cres-t能解决这个问题,那么具体原理是什么呢?
repression domain SRDX---cres-t原理是什么呢?
研究转录因子功能,转录因子缺失表达时,由于转录因子同源家族基因功能补偿,使得表型中很难辨别,cres-t能解决这个问题,那么具体原理是什么呢?
repression domain SRDX---cres-t原理是什么呢?研究转录因子功能,转录因子缺失表达时,由于转录因子同源家族基因功能补偿,使得表型中很难辨别,cres-t能解决这个问题,那么具体原理是什么呢?
CRES-T (chimeric repressor gene–silencing technology)
It dominantly suppresses the transcription of genes regulated by the transcription factor of interest and is a useful technique for functional analysis of transcription factors
For example, you can generate a CRES-T mutant library for transcription factors thta you are interested at, and use the library to identify the funcation/role of these transcription factors.
It involves only the expression of a fusion protein (a transcriptional activator with a repression domain). The repressive activity of the chimeric repressor can overcome the activities of endogenous and functionally redundant transcription factors, with resultant induction of a dominant negative phenotype.
I think this paper may help you
Hiratsu et al., 2003 K. Hiratsu, K. Matsui, T. Koyama and M. Ohme-Takagi, Dominant repression of target genes by chimeric repressors that include the EAR motif, a repression domain, in Arabidopsis, Plant J. 34 (2003), pp. 733–739
Hope these help.