Many economical important primary metabolites such as amino acids,in particular L-lysine using Corynebacterium have been successfully overproduced by mutation.(a) Give your idea on how auxotropic mutant for lysine production could be accomplished and
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Many economical important primary metabolites such as amino acids,in particular L-lysine using Corynebacterium have been successfully overproduced by mutation.(a) Give your idea on how auxotropic mutant for lysine production could be accomplished and
Many economical important primary metabolites such as amino acids,in particular L-lysine using Corynebacterium have been successfully overproduced by mutation.
(a) Give your idea on how auxotropic mutant for lysine production could be accomplished and (b) Given currently developed molecular cloning technique,how would you compare traditional mutation technique in terms of merits and drawback?You may compare this with genetically modified foods.
Many economical important primary metabolites such as amino acids,in particular L-lysine using Corynebacterium have been successfully overproduced by mutation.(a) Give your idea on how auxotropic mutant for lysine production could be accomplished and
给你汉语答案吧.
1．关于营养缺陷型菌株生产赖氨酸的问题.
在许多微生物中,可用天冬氨酸作原料,通过分支代谢途径合成出赖氨酸、苏氨酸和甲硫氨酸.但在代谢过程中,一方面由于赖氨酸对天冬氨酸激酶有反馈抑制作用,另一方面,由于天冬氨酸除用于合成赖氨酸外,还要作为合成甲硫氨酸和苏氨酸的原料,因此,在正常细胞内,就很难累积较高浓度的赖氨酸.工业上利用谷氨酸棒杆菌的高丝氨酸缺陷型菌株作为赖氨酸的发酵菌种.由于它不能合成苏氨酸脱氢酶,故不能合成高丝氨酸,也不能产生苏氨酸和甲硫氨酸,在补以适量高丝氨酸的条件下,可在较高糖浓度和铵盐的培养基上,产生大量的赖氨酸.
2．传统经典的诱变生产(赖氨酸）技术和分子克隆技术之优缺点比较
赖氨酸的高产菌株已通过经典的诱变法获得,但要再提高这些诱变株的产量就很困难.
而分子克隆技术生产,产量大,节约成本.但也会带来一些问题.
许多经济重要的初级代谢产物，如氨基酸，尤其是L -赖氨酸利用棒状杆菌已成功地过量的突变。
（一）给您的想法如何auxotropic突变为赖氨酸生产可以完成，而且（ b ）鉴于目前发达国家分子克隆技术，你将如何比较传统突变技术方面的优点和缺点？您可能会比较与转基因食品。
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许多经济重要的初级代谢产物，如氨基酸，尤其是L -赖氨酸利用棒状杆菌已成功地过量的突变。
（一）给您的想法如何auxotropic突变为赖氨酸生产可以完成，而且（ b ）鉴于目前发达国家分子克隆技术，你将如何比较传统突变技术方面的优点和缺点？您可能会比较与转基因食品。
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很多重要的经济的初级代谢产物，像氨基酸，尤其是赖氨酸用于棒状杆菌属已经由于变异而被成功地过度生产.