请帮我解释一下overlapping subclone的意思,谢谢!那请帮我翻译一句话：Using overlapping subclones prepared by nested deletion, the ORF of p1Dy10.1 was completely sequenced.十分感谢！

请帮我解释一下overlapping subclone的意思,谢谢!那请帮我翻译一句话：Using overlapping subclones prepared by nested deletion, the ORF of p1Dy10.1 was completely sequenced.十分感谢！
请帮我解释一下overlapping subclone的意思,谢谢!
那请帮我翻译一句话：Using overlapping subclones prepared by nested deletion, the ORF of p1Dy10.1 was completely sequenced.十分感谢！

请帮我解释一下overlapping subclone的意思,谢谢!那请帮我翻译一句话：Using overlapping subclones prepared by nested deletion, the ORF of p1Dy10.1 was completely sequenced.十分感谢！
从意思上讲是“重叠亚克隆”,是指一组克隆/质粒中,每一个克隆的插入基因序列和另一个的序列间有重叠部分.
可以用在测序上,当一组插入序列都被测序之后,可以通过计算机参考重叠序列将所有结果拼接成一条大的完整序列.
依靠嵌套缺失法获得的重叠亚克隆,p1Dy10.1的开放读码框已被完整测序

重叠亚克隆

是亚克隆重叠（交错）的意思，应该是克隆形式的一种。