生物英文翻译2GUS expression was absent in the progeny of a cross betweentransgenic plants carrying 35S::GUS-SPL3 and plants carrying35S::miR156a (Fig. 2B). Progeny from a cross between 35S::GUSSPL3mand 35S::miR156a had high levels of GUS activi

生物英文翻译2GUS expression was absent in the progeny of a cross betweentransgenic plants carrying 35S::GUS-SPL3 and plants carrying35S::miR156a (Fig. 2B). Progeny from a cross between 35S::GUSSPL3mand 35S::miR156a had high levels of GUS activi
生物英文翻译2
GUS expression was absent in the progeny of a cross between
transgenic plants carrying 35S::GUS-SPL3 and plants carrying
35S::miR156a (Fig. 2B). Progeny from a cross between 35S::GUSSPL3m
and 35S::miR156a had high levels of GUS activity, and there
was no significant difference between the amount of GUS activity
in these plants (35,821±2815 pmol 4-MU/minute·\3g protein) and
the progeny of a control cross between 35S::GUS-SPL3m and plants
carrying an empty vector (33,599±2109 pmol 4-MU/minute·\3g
protein). These results demonstrate that the SPL3 mRNA is a direct
target of miR156 and show that the mutations we introduced in the
miR156 target site completely block the activity of this miRNA.
Transgenic plants carrying 35S::SPL3 were essentially normal;
they flowered at nearly the same time and with the same number of
leaves as wild-type plants, and had the same pattern of trichomeproduction (Fig. 2C,D). By contrast, plants expressing SPL3 with
mutated miR156 target site (35S::SPL3m) or SPL3 lacking the 3\2
UTR (35S::SPL3\5), flowered early, had a significantly reduced
number of juvenile, adult and cauline leaves (Fig. 2C-E). The first
two leaves of transgenic plants had a shorter petiole and a more acute
leaf base than wild-type leaves (Fig. 2E); these features are typical
of adult leaves. These results indicate that SPL3 promotes both
vegetative and reproductive phase change and is normally repressed
by miR156.
Plants constitutively expressing the a, b, c, d, e and f isoforms of
miR156 were generated by transforming wild-type plants with
constructs containing 0.3-0.8 kb of intergenic genomic sequence
under the regulation of the 35S promoter (Fig. 3A). All of these
transgenic lines had a similar phenotype (Fig. 3B), and resembled
the 35S::156b transgenics described by Schwab et al. (Schwab et
al., 2005). Thus, all these loci are capable of producing miR156although whether they are all transcribed in vivo is unknown.
A detailed phenotypic analysis was performed on plants
overexpressing miR156a. In continuous light, transgenic plants
had a significantly larger number of leaves without abaxial
trichomes (n=24; P

生物英文翻译2GUS expression was absent in the progeny of a cross betweentransgenic plants carrying 35S::GUS-SPL3 and plants carrying35S::miR156a (Fig. 2B). Progeny from a cross between 35S::GUSSPL3mand 35S::miR156a had high levels of GUS activi
缺席的GUS表达的转基因植物杂交后代继承的35S::的GUS-spl3携带动植物的35S::mir156a(图 二号B).杂交后代的35S::gusspl3m的35S和::GUS活性mir156a量偏高,并且没有明显的差异额在这些植物GUS活性(35821+2815小于4-mu/minute●G蛋白) 对照两岸关系后代的35S::的GUS-spl3m动植物携带空载体(33599+2109小于4-mu/minute●G蛋白).这些结果表明,基因是直接spl3mir156和目标表明,我们实行的突变 在现场mir156目标完全堵住这一自然资源活动.转基因植物携带的35S::spl3基本上正常; 他们差不多同时开花,以同样数目为叶野生型植物,丁有着同样的模式trichomeproduction(图二号丙、丁).相比之下,与植物表达spl3变种mir156目标地皮(的35S::spl3m)或缺spl3300翻译(的35S::spl3) 早开花,多少年了大幅度降低,茎、叶成年(图二号E型).头两个转基因植物叶片和叶柄有短叶基地更加尖锐,比野生型叶片 (图2E)款; 这些特点是典型的成年叶片.这些结果表明,营养和生殖spl3促进双方相变及mir156通常被打压.植物组成的一个表述,乙、丙、丁、 E和F亚型mir156被转化产生野生型植物基因构造含有罩杯字节间隔 调控下的序列35S启动(图3A)款.所有这些线路也有类似的基因表型(图乙) 而近似的35S的::认为转156b形容施瓦布等.(施瓦布等.,2005).因此,这些基因能产生mir156although抄写体内是否都还是未知数.详细分析型植物过量mir156a术.连续轻,转基因植物具有明显较多叶片无离心atrichomes(24例; 磷

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