英语翻译Proteinase K,a non-specific proteinase,is an excellent choice to degrade the proteins,since the sequence and structure of the fibrous protein of the otolith remain unknown.Only the amino acid composition of the otoliths has been determine

英语翻译Proteinase K,a non-specific proteinase,is an excellent choice to degrade the proteins,since the sequence and structure of the fibrous protein of the otolith remain unknown.Only the amino acid composition of the otoliths has been determine
英语翻译
Proteinase K,a non-specific proteinase,is an excellent choice to degrade the proteins,since the sequence and structure of the fibrous protein of the otolith remain unknown.
Only the amino acid composition of the otoliths has been determined (Degens et al.,
1969; Dunkelberger et al.,1980; Asano & Mugiya,1993).The enzyme cleaves proteins at
the A–X bond,where A is aliphatic,aromatic or hydrophobic amino acid and X is any
amino acid.It can sever proteins into free amino acids when used in large quantities over
long incubation periods.
The largest among the three pairs of the otoliths,the sagitta,was used in these
experiments.The otoliths were first embedded in resin (L.R.white) and polished until
the core was exposed on the sectioned surface.Next,the otoliths were digested with
c.0·2 ml proteinase K buVer (PKb),in 1·5-ml Eppendorfs at 45) C with gentle shaking.
To shorten the reaction time,sodium dodecyl sulphate (SDS) was added to the buVer.
SDS can elevate the activity of proteinase K by as much as sevenfold (Hilz et al.,1975).
Nevertheless,the reaction time depended on the species and the temperature.Every
few minutes or hours,otoliths were extracted from the Eppendorfs to examine the
etching results under the light microscope until the optimal etching conditions were
reached (Table I).Other ingredients used in the PKb were diluted from stock solution
(Table I).After the digestion by PKb,the otoliths were dried in the oven and coated with
a layer of gold for SEM (Hitachi S2300) observation.Sometimes,if SDS crystallized on
the surface of otoliths,75% alcohol could dissolve the same away easily.

英语翻译Proteinase K,a non-specific proteinase,is an excellent choice to degrade the proteins,since the sequence and structure of the fibrous protein of the otolith remain unknown.Only the amino acid composition of the otoliths has been determine
蛋白酶K,未指明的蛋白酶,是贬低proteins,since的一个优秀选择序列,并且otolith的纤维状蛋白质的结构依然是未知.otoliths的氨基酸组成确定了Only (等Degens,
1969; Dunkelberger等,1980年; asano & Mugiya 1993).酵素劈开蛋白质在
the A–X债券,A是脂肪族的,芳香或疏水氨基酸和X是其中任一
氨基酸.它可能切断蛋白质入游离氨基酸,当大量地使用
long潜伏期.
The最大在otoliths的三个对之中,sagitta,用于这些
experiments.otoliths在树脂(L.R.白色)首先被埋置了并且被擦亮了直到
the核心在被区分的表面被暴露了.其次,otoliths消化了与
c.0·2 ml蛋白酶K buVer (PKb),在1·在45)与柔和震动的C的5ml Eppendorfs.
To缩短反应时间,钠十二烷基的硫酸盐(SDS)增加了到buVer.
SDS可能举起蛋白酶K的活动和七倍一样多(等Hilz,1975).
Nevertheless,反应时间取决于种类和温度.每
few分钟或几小时,otoliths从Eppendorfs被提取审查
etching收效在光学显微镜下,直到优选的蚀刻条件是
reached (表I).用于PKb的其他成份从储备溶液被稀释了
(表I).在由PKb的消化以后,otoliths在烤箱被烘干了并且涂用 金子a层数SEM (日立S2300)观察的.有时,如果SDS结晶了 otoliths the表面,75%酒精能溶化同样容易地.

蛋白酶K ，非特异性蛋白酶，是一个很好的选择降解的蛋白质，由于顺序和结构的纤维蛋白的耳石仍下落不明。
只有氨基酸组成的耳石已确定（德根斯等人，
1969年; dunkelberger等人， 1980年; ＆浅野麦屋， 1993年） 。酶蛋白在cleaves
该一- X的债券，如果是脂肪族，芳香族或疏水性氨基酸和X是任何
氨基酸。它可以割断的蛋白质变成游离氨基...

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蛋白酶K ，非特异性蛋白酶，是一个很好的选择降解的蛋白质，由于顺序和结构的纤维蛋白的耳石仍下落不明。
只有氨基酸组成的耳石已确定（德根斯等人，
1969年; dunkelberger等人， 1980年; ＆浅野麦屋， 1993年） 。酶蛋白在cleaves
该一- X的债券，如果是脂肪族，芳香族或疏水性氨基酸和X是任何
氨基酸。它可以割断的蛋白质变成游离氨基酸含量时，所用的大量超过
长期潜伏期。
最大的之间的三对该耳石，三趾跳鼠，用在这些
实验。该耳石首次嵌入在树脂表（ LR白色）和抛光，直到
核心是暴露在分段表面。接下来，耳石消化
长0.2毫升蛋白酶K buver （蛋白激酶B ） ，在1.5毫升eppendorfs在45 ） c与温柔摇头。
以缩短反应时间，十二烷基硫酸钠（ SDS ）的被添加到buver 。
的SDS能提高活性蛋白酶K由高达七倍（ hilz等人， 1975年） 。
不过，反应时间依赖于物种和温度。每个的
几分钟或几小时，耳石提取从eppendorfs研究
蚀刻下的结果，光镜，直至最佳蚀刻条件
达成的（见表一） 。其他配料使用，在蛋白激酶B稀释从原液
（见表一） 。后消化蛋白激酶B ，耳石干燥，在烤箱和包覆
一层黄金的扫描电镜（日立s2300 ）观察。有时候，如果对结晶的SDS
表面耳石， 75 ％酒精可以解散，同时远离容易。

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