英语翻译VDAC2 and aldolase A identified as membrane proteins of K562cells with increased expression under iron deprivationAbstract：We have shown previously that iron deprivationsignificantly stimulates the uptake of non-transferrinferric iron f

英语翻译VDAC2 and aldolase A identified as membrane proteins of K562cells with increased expression under iron deprivationAbstract：We have shown previously that iron deprivationsignificantly stimulates the uptake of non-transferrinferric iron f
英语翻译
VDAC2 and aldolase A identified as membrane proteins of K562
cells with increased expression under iron deprivation
Abstract：We have shown previously that iron deprivation
significantly stimulates the uptake of non-transferrin
ferric iron from ferric citrate by erythroleukemia K562
cells and that this stimulation depends on protein synthesis.
However,we have not detected increased expression of any
known iron transport protein (Kovar J.et al.(2006) Blood
Cells Mol Dis 37:95–99).Therefore,in order to identify
membrane proteins of K562 cells with increased expression
under iron deprivation,we employed the isolation of
membrane proteins by two-phase partitioning system,
protein separation by high-resolution 2D electrophoresis,
computer differential analysis,and tandem mass spectrometry.
Employing these techniques we identified two
proteins with statistically significant upregulation,i.e.,
aldolase A (ALDA) and voltage-dependent anion channel 2
(VDAC2).The upregulation of aldolase A and VDAC2 in
K562 cells under iron deprivation was also confirmed by western blot analysis.This is the first time when the control
of aldolase A and VDAC2 levels by iron status of the cell is
demonstrated.

英语翻译VDAC2 and aldolase A identified as membrane proteins of K562cells with increased expression under iron deprivationAbstract：We have shown previously that iron deprivationsignificantly stimulates the uptake of non-transferrinferric iron f
VDAC2和醛甲确定膜蛋白的K562
细胞表达增加下铁剥夺
摘要：我们已经表明以前铁剥夺
大大刺激了摄取的非转
铁铁铁柠檬酸的白血病K562细胞
细胞和刺激,这取决于蛋白质的合成.然而,我们没有发现增加任何的表达
众所周知铁转运蛋白（可伐学者等.（ 2006 ）血
细胞与分子杂志37:95-99 ） .因此,为了确定
膜蛋白的K562细胞表达增加
铁剥夺下,我们采用了分离
膜蛋白的两相分配制度,
蛋白质分离的高分辨率二维电泳,
计算机鉴别分析,并串联质谱.
采用这些技术,我们确定了两个
蛋白质与统计学上调,即：
醛甲（阿尔达）和电压依赖阴离子通道2
（ VDAC2 ） .该上调醛缩酶A和VDAC2在
K562细胞剥夺下铁也证实了免疫印迹分析.这是第一次当控制
的醛缩酶A和VDAC2水平的铁营养状况的细胞是
证明.