英文翻译——物理专业4请帮忙翻译这段文字:Fluorescence Lifetime Measurements. Fluorescence lifetimes were measured using a custom-built far-field epifluorescence microscope coupled to a spectrometer and time-gated intensified charg
来源:学生作业帮助网 编辑:六六作业网 时间:2024/12/19 14:52:26
英文翻译——物理专业4请帮忙翻译这段文字:Fluorescence Lifetime Measurements. Fluorescence lifetimes were measured using a custom-built far-field epifluorescence microscope coupled to a spectrometer and time-gated intensified charg
英文翻译——物理专业4
请帮忙翻译这段文字:Fluorescence Lifetime Measurements. Fluorescence lifetimes were measured using a custom-built far-field epifluorescence microscope coupled to a spectrometer and time-gated intensified charge-coupled device (CCD) camera (Imager QE, LaVision GmbH, Goettingen, Germany). The microscope setup permitted use of very small excitation powers (~30 flW) while maintaining a strong photoemission signaL The excitation light source was coupled to the microscope collection axis using a 400 nm dichroic filter and a 0.3 NA lOx objective (Nikon, Tokyo, Japan) placed after the dichroic filter was used to focus the excitation light onto the sample and collect the emitted fluorescence signal. An additional long pass filter inserted after the dichroic mirror was used to eliminate residual signal from the laser line and transmit only relevant (PL) signals onto the CCD detector. A pulsed GaN diode laser (414 nm, 5 MHz, 90 ps full width at half-maximum, model LDH400, PicoQuant GmbH, Berlin, Germany) was used to excite the sample. The overall instrument response time was limited to 500 ps by the CCD image intensifier microchannel plate. Data were collected at room temperature using a 10 mm optical path fluorescence cell filled with 3 mL of QD conjugates in buffer solution, as described above. Raw intensity data were obtained as functions of time and wavelength using DaVis software (LaVision GmbH).
翻译软件要是能翻得通顺,你以为我还贴到这干什么,谢谢大家.
英文翻译——物理专业4请帮忙翻译这段文字:Fluorescence Lifetime Measurements. Fluorescence lifetimes were measured using a custom-built far-field epifluorescence microscope coupled to a spectrometer and time-gated intensified charg
我学电子信息工程的,文献翻译很多靠谷歌翻译,现在谷歌翻译已经做的相当不错了,在它的基础上修改还是很节约时间的.
你物理我不懂,一下为谷歌段落翻译,希望对你有帮助
荧光寿命测量. 荧光寿命测定采用定制的远场萤光显微镜加上一个分光计和时间选通增强电荷耦合器件(CCD)摄像头(成像量化宽松政策,LaVision有限公司,哥廷根,德国). 该显微镜设置允许非常小的激励权力(~30 flW),同时保持一个强大的光电子信号的激发光源耦合至显微镜收集轴采用双色的400纳米过滤器和一个0.3不适用液氧目标(尼康,东京,日本使用)放置过滤器后,采用双色聚焦到样品的激发光,并收集所发射的荧光信号. 另外一个长传后插入滤波器分色镜是用来消除残留的激光信号传输线唯一相关(特等)到CCD探测器的信号. 一个脉冲氮化镓半导体激光(414纳米,5兆赫,90 ps的半最大,模型LDH400,PicoQuant有限公司,柏林,德国全宽)被用来激发样品. 整体仪器的响应时间被限制在500人的CCD图像增强器微通道板的PS. 收集资料,在室温下使用10毫米光学路径细胞荧光量子点复合物3在缓冲溶液如上所述,毫升填补. 原始强度数据,得到的时间的功能和使用波长戴维斯软件(LaVision有限公司).