目的基因片段与载体DNA连接的主要形式呢?Excuse me,could you translate 1.restriction enzyme ligation.2.TOPO TA3.Gateway system4.blunt end ligation into Chinese?Because my English is very poor!
来源:学生作业帮助网 编辑:六六作业网 时间:2024/12/24 13:58:05
目的基因片段与载体DNA连接的主要形式呢?Excuse me,could you translate 1.restriction enzyme ligation.2.TOPO TA3.Gateway system4.blunt end ligation into Chinese?Because my English is very poor!
目的基因片段与载体DNA连接的主要形式呢?
Excuse me,could you translate 1.restriction enzyme ligation.2.TOPO TA3.Gateway system4.blunt end ligation into Chinese?Because my English is very poor!
目的基因片段与载体DNA连接的主要形式呢?Excuse me,could you translate 1.restriction enzyme ligation.2.TOPO TA3.Gateway system4.blunt end ligation into Chinese?Because my English is very poor!
1、黏性末端DNA的连接:单酶切黏性末端、双酶切黏性末端、不规则黏性末端
2、平末端DNA的连接:直接连接法、同聚物加尾法、衔接物连接法、DNA接头连接法、PCR法引入酶切位点的连接
1、限制性内切酶链接 2、TOPO克隆(一种克隆技术) 3、网关系统(门户系统) 4、平端连接
1. restriction enzyme ligation.
2. TOPO TA
3. Gateway system
4. blunt end ligation