英语翻译请各位不要用翻译软件翻,这个有点麻烦了,The activity of M.echinospora proteases was investigated over a broad pH range (pH 5 –9.5) in the following buffers:150 mM sodium citrate (pH 3 –4),150 mM sodium acetate/acetic a

英语翻译请各位不要用翻译软件翻,这个有点麻烦了,The activity of M.echinospora proteases was investigated over a broad pH range (pH 5 –9.5) in the following buffers:150 mM sodium citrate (pH 3 –4),150 mM sodium acetate/acetic a
英语翻译
请各位不要用翻译软件翻,这个有点麻烦了,
The activity of M.echinospora proteases was investigated over a broad pH range (pH 5 –9.5) in the following buffers:150 mM sodium citrate (pH 3 –4),150 mM sodium acetate/acetic acid (4 –6.5),sodium phosphate (6.5 –7.5),Tris/HCl (pH 7.5 –9).Protease activity was found to have an optimum of pH 7 throughout growth (data not shown).No differences were observed in the pH optima of the proteases from different media,or culture conditions.An indication of proteolytic class was determined by incubation of culture supernatants with a range of protease inhibitors,according to the method of Dunn (1989).Protease inhibitor studies were conducted on samples taken from the stationary phase of YEME grown cultures.The extracellular proteases of M.echinospora were subject to significant inhibition of proteolytic activity with TLCK (Tosyl-lysyl-chloromethyl-ketone; an inhibitor of Trypsinlike serine proteases,100 mM),TPCK (Tosyl-phenylalanyl-chloromethyl-ketone; an inhibitor of chymotrypsin-like serine proteases,100 mM),E-64 (an inhibitor of cysteine proteases,1 mM),and Pepstatin (an inhibitor of aspartic proteases,1 mM).Inhibition of protease activity by EDTA (an inhibitor of metalloproteases,100 mM) was less significant.These data suggest that M.echinospora produces at least three types of proteases during growth (Table 2).
To examine the possible physiological role of the proteolytic enzymes,protease inhibitors were applied onto the surface of growing cultures (on filter papers impregnated with the inhibitor).Sporulation was inhibited by the application of TLCK and TPCK,established by the absence of the culture darkening (Figure 2) and confirmed by microscopic impression mounts of the culture surface (data not shown).This suggests a link between a serine protease and morphological differentiation in M.echinospora.Given that morphological differentiation in M.echinospora was accompanied by the production of extracellular proteolytic enzymes,and that differentiation can be inhibited by the application of protease inhibitors,it is reasonable to assume they play some role in sporulation in this organism.It has been shown that extracellular proteolytic enzymes in bacteria may be synthesised in response to the presence of an inducing substrate within the medium,or when growth conditions are suboptimal for growth.In Bacillus protease production and sporulation have been linked (Ochi 1985) and genes having pleiotropic effects on both processes have been identified (Piggot and Coote 1976).

英语翻译请各位不要用翻译软件翻,这个有点麻烦了,The activity of M.echinospora proteases was investigated over a broad pH range (pH 5 –9.5) in the following buffers:150 mM sodium citrate (pH 3 –4),150 mM sodium acetate/acetic a
在以下几种缓冲液中（或基于以下几种缓冲液）,（我们）从一个较大的PH值范围内对棘孢小单孢菌蛋白（水解）酶的活性进行探讨：150mm柠檬酸钠（PH 3-4）,150mm乙酸钠/乙酸（PH 4-6.5）,磷酸钠（PH -6.5-7.5）,Tris/盐酸（PH 7.5-9）.发现在整个培养过程中（未显示的数据）,当pH值为7 时,蛋白酶具有最佳活性.在不同基质中或不同培养条件下培养的蛋白酶,并没有发现它们的最适PH值有什么差异.根据1989年耿式实验法,大致的水解程度是由培养的上清液的繁殖速度决定的,这些上清液中含有大量的蛋白酶抑制剂.从YEME（一种液体培养基）成熟培养物的稳定期提取样本,对蛋白酶抑制剂进行研究.在加有TLCK（甲苯硫酰基-赖氨酸-氯甲基-酮；一种类似胰蛋白酶的丝氨酸蛋白酶抑制剂,100mm）,TPCK（甲苯硫酰基-苯丙氨酰基-氯甲基-酮；一种类似胰凝乳蛋白酶的丝氨酸蛋白酶抑制剂,100mm）,E-64(一种半胱氨酸蛋白酶抑制剂,1mm),胃酶抑素（一种天冬氨酸蛋白酶抑制剂,1mm）的培养液中,棘孢小单孢菌细胞外的蛋白酶的水解活动明显受到抑制.EDTA（一种金属蛋白酶抑制剂,100mm）对水解活动的抑制作用就不是那么明显.这些数据表明棘孢小单孢菌在生长过程中至少能合成3中蛋白酶（表2）.
主要是为了检验在培养物（在一张注满抑制剂的滤纸上）快速生长的过程中,蛋白水解酶和蛋白酶抑制剂可能对其表面产生的作用.孢子的形成会受到TLCK和TPCK的抑制,移除培养基质上的染色剂能促使孢子的形成,通过培养物表面（未显示的数据）在显微镜下的印模封片能证实这一点.这就暗示了丝氨酸蛋白酶与棘孢小单孢菌的形态分化有某种联系.考虑到棘孢小单孢菌发生形态分化的同时,还伴随着细胞外蛋白水解酶的合成,而且这种分化能被蛋白酶抑制剂抑制,则我们假设蛋白水解酶和蛋白酶抑制剂在这个有机体中对孢子的形成能起某种作用,是十分合理的.这已经表明了一个结论：细菌细胞外的蛋白水解酶的合成,可能是由于培养基质中存在一种诱导酶,或者生长条件没有达到最佳水平.在杆菌中,蛋白酶的合成和孢子的形成已被验证有某种联系（由Ochi于1985年证实）,并且两个过程中的多效基因已经被鉴别（Piggot 和Coote于1976年完成） .
花了一晚上,水平有限,只能翻译到这种程度了.对于culture这个单词在文中的意思,不是很确定,词典上翻译为“培养”、“培养物”.但有的地方翻译为这两个意思似乎说不通,所以按自己的意思翻译了.