英语翻译建立抗癌药物紫杉醇检测的薄层层析(TLC)方法,可以用于紫杉醇产生菌株的快速筛选.将紫杉醇标准品或内生真菌发酵液的萃取物,点于10-40 µm粒度硅胶的薄层层析板,以氯仿-甲醇液
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英语翻译建立抗癌药物紫杉醇检测的薄层层析(TLC)方法,可以用于紫杉醇产生菌株的快速筛选.将紫杉醇标准品或内生真菌发酵液的萃取物,点于10-40 µm粒度硅胶的薄层层析板,以氯仿-甲醇液
英语翻译
建立抗癌药物紫杉醇检测的薄层层析(TLC)方法,可以用于紫杉醇产生菌株的快速筛选.将紫杉醇标准品或内生真菌发酵液的萃取物,点于10-40 µm粒度硅胶的薄层层析板,以氯仿-甲醇液作为展开剂进行层析,然后立即使用含有1%香草醛的浓硫酸液喷雾显色,观察紫杉醇的特征性蓝色斑点.紫杉醇的分离检测效果较好,借助紫杉醇的薄层层析显色反应可以筛选到产生紫杉醇的内生真菌.应用薄层层析技术对内生真菌的发酵产物进行检测,能够简便、准确的筛选到紫杉醇产生菌株,并且可以初步推测菌株的紫杉醇产生能力；对于产生紫杉醇的药用真菌筛选研究,比HPLC方法更加高效.
英语翻译建立抗癌药物紫杉醇检测的薄层层析(TLC)方法,可以用于紫杉醇产生菌株的快速筛选.将紫杉醇标准品或内生真菌发酵液的萃取物,点于10-40 µm粒度硅胶的薄层层析板,以氯仿-甲醇液
Establishment of anticancer drug taxol detection of thin-layer chromatography ( TLC ) method, can be used for rapid screening of taxol producing strains. The paclitaxel standard or endophytic fungus fermentation liquid extract, point to m10-40particle size silica gel thin layer chromatography plate, with chloroform - methanol liquid chromatography as the expansion agent, and then immediately use containing1% vanillin sulphuric acid liquid spray color, study on paclitaxel characteristic blue spots. Taxol separation detection results, with the aid of taxol by thin layer chromatography and color reaction can be filtered to produce taxol endophytic fungi. Application of thin layer chromatography technique on endophytic fungi fermentation products were detected, which can conveniently, accurately to the screening of taxol producing strains, and strains can be speculated that taxol producing ability; to produce taxol medicinal fungi screening study, more efficient than HPLC method.
也就这样了,太乱了