英语翻译MG：孔雀石绿LMG：隐色孔雀绿3 .Results and discussionStandard curves of the regressed concentration points gave squared correlation coefficients better than 0.9999 for each MG and LMG separate and as a mixture.The time interval

英语翻译MG：孔雀石绿LMG：隐色孔雀绿3 .Results and discussionStandard curves of the regressed concentration points gave squared correlation coefficients better than 0.9999 for each MG and LMG separate and as a mixture.The time interval
英语翻译
MG：孔雀石绿
LMG：隐色孔雀绿
3 .Results and discussion
Standard curves of the regressed concentration points gave squared correlation coefficients better
than 0.9999 for each MG and LMG separate and as a mixture.The time interval between the moment of spiking and moment of sample processing had a dramatic influence on the recovery of MG,viz.81,63,60,54 and 45% for intervals of 1,15 and 30 min,and 1 and 2 h,respectively.Since no corresponding signal for LMG was observed,MG was apparently not reduced in vitro into LMG during this incubation.In contrast,little effect was observed on the recovery of LMG,viz.100,96,93,93 and 95% for intervals of 1,15 and 30 min,and 1 and 2 h,respectively.Furthermore,inspection of the liquid extraction process learned that homogenization using ultraturrax or the household blender gave similar and best recoveries of the MG residues compared to ultrasonification and no support at all (results not shown).
A close inspection of chromatograms of processed fish fillet samples,showed significant satellite peaks (up to 15% of the parent peak area) suggesting degradation of MG and especially of LMG.This degradation is most probably de-methylation of the dye [10–12].Comparison of the analysis of spiked samples with that of the standards revealed that this breakdown originated partly from the working-up procedure,as suggested earlier [10].In this study,it was found that addition of TMPD and ascorbic acid to the laboratory samples could largely reduce this degradation in all tested tissues.
The accuracy of the test was optimized in the method development for the lipophilic LMG
derivative and not for the hydrophilic parent molecule,as LMG is the prevalent residue observed long after administration of MG [13,14].In tracing illegal use of MG,LMG seems therefore the best indicator molecule in analysis strategies,as suggested before [3].Recoveries determined at various levels in catfish,eel,trout,turbot and south-Asian prawns reflected this choice (Table 1).Namely,whereas in the case of MG,recoveries ranged from 43.8±1.9% (at 5 mg/kg in rainbow trout) to 67.7% (at 100 mg/kg in eel),recoveries of LMG were between 86±15% (at 2.5 mg/kg in prawn) and 105614% (at 2.5 mg/kg in eel).Recoveries from non-processed salmon fillets were in the same range,namely 57±4 and 95±5% for MG and LMG,respectively.These recoveries from smoked and canned salmon were 72±4% (MG) /84±2% (LMG) and 87±8% (MG) /94±2% (LMG),respectively.
The limit of detection (LOD) was determined on the basis of the response of blank samples plus three times the standard deviation (SD) compared with that of spiked samples,and was 1 mg/kg for both MG and LMG.This sensitivity is in accordance with those reported for similar HPLC analysis approaches,in which LODs were reported at 2 mg/kg (MG and LMG) [3,8],and at 2 mg/kg (MG) and 1 mg/kg (LMG) [15].
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英语翻译MG：孔雀石绿LMG：隐色孔雀绿3 .Results and discussionStandard curves of the regressed concentration points gave squared correlation coefficients better than 0.9999 for each MG and LMG separate and as a mixture.The time interval
3. 结果和讨论
对于MG和LMG分开和混合溶液的一些递减浓度的点回归得到的标准曲线给出优于0.9999的二次方相关系数.在出峰时刻和样品处理时刻之间的时间间隔对MG的回收有着戏剧性的影响,间隔为1、15、30分钟、1小时和2小时的回收率分别为81%、63%、60%、54%和45%.由于对于LMG来说没有观察到相关性特征,很明显MG在试管中的温浴（incubation这里通常指在恒温水浴箱中放置）过程中没有被还原成LMG.相反,对LMG的回收率的影响很小,间隔为1、15、30分钟、1小时和2小时的回收率分别为100%、96%、93%、93%和95%.此外,对液相提取过程的考察看出用ultraturrax或家用混合器制作的允浆液与超声作用和完全没有支持的情况相比给出相似的且最好的MG残留物的回收率（结果未显示）.
对加工的鱼片样品液相图谱的仔细考察,看出有明显的伴峰（达到主峰面积的50%）,提示MG和（特别是）LMG的降解作用.这种降解作用最有可能使这种染料的脱甲基作用 [10–12].刺样品的分析与标准标准样品相比揭示出这种降解部分上源于前期所提到的整理过程[10].在此研究中,发现将TMPD和抗坏血酸加入实验室样品可以在所有测试组织中极大地减少此降解作用.这种检测的精确度在对脂溶性的LMG衍生物而不是脂溶性的前体分子的方法学研究中达到优化,因为LMG在对MG长时间处理之后所观察到的主要残留物[13,14].因此,如前面所提出的,对于追踪MG的非法使用来说,在分析策略上LMG似乎是最好的指示剂[3].在各种水平的鲶鱼、美洲鳗、鲑鱼和南亚对虾中确定的回收率反映出这种选择（表1）.也就是说,对于MG来说,回收率的范围是从43.8±1.9%（5 mg/kg在虹鳟鱼中）至67.7%（100 mg/kg在美洲鳗中）,而LMG的回收率范围是在86±15%（2.5 mg/kg在对虾中）到105614%（2.5 mg/kg在美洲鳗中）.未经处理的鲑鱼片的回收率是在相同的范围,即对于MG和LMG来说分别为57±4和95±5%.这些回收率从熏制和罐装的鲑鱼的回收率结果分别为72±4%（MG）/84±2%（LMG）和 87±8%(MG)/94±2% (LMG).
在空白样品的响应值加上与刺过样品相比三倍标准误（SD）的基础上,确定了检测限（LOD）,对于MG和LMG两者都是1 mg/kg.这种敏感度与那些相似的HPLC分析方法的报告是相符合的,这些报告中所报告LOD是2 mg/kg（MG和LMG）[3,8]、2 mg/kg（MG）和1 mg/kg（LMG）[15].