英语翻译After the membranes were blocked in 5% skimmed milk in PBS-T containing 0.05% Tween 20 for 2 h at RT,they were incubated with antibodies to mTOR,p70s6K,p-P70s6K,4EBP1,p-4EBP1 and b-actin diluted in 1% skimmed milk in PBS-T,respectively,at

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英语翻译Afterthemembraneswereblockedin5%skimmedmilkinPBS-Tcontaining0.05%Tween20for2hatRT,theywereincuba

英语翻译After the membranes were blocked in 5% skimmed milk in PBS-T containing 0.05% Tween 20 for 2 h at RT,they were incubated with antibodies to mTOR,p70s6K,p-P70s6K,4EBP1,p-4EBP1 and b-actin diluted in 1% skimmed milk in PBS-T,respectively,at
英语翻译
After the membranes were blocked in 5% skimmed milk in PBS-T containing 0.05% Tween 20 for 2 h at RT,they were incubated with antibodies to mTOR,p70s6K,p-P70s6K,4EBP1,p-4EBP1 and b-actin diluted in 1% skimmed milk in PBS-T,respectively,at RT for 2 h,.

英语翻译After the membranes were blocked in 5% skimmed milk in PBS-T containing 0.05% Tween 20 for 2 h at RT,they were incubated with antibodies to mTOR,p70s6K,p-P70s6K,4EBP1,p-4EBP1 and b-actin diluted in 1% skimmed milk in PBS-T,respectively,at
大致是这样：括号内是我解释用的字,原文中无.
在室温下,膜被5%的脱脂牛奶PBS-T 溶液洗2小时,（PBS是磷酸缓冲溶液,里面加0.05%的tween 20用来做表面活性剂）. 他们在室温条件下,被分别放在含有抗体 mTOR, p70s6K, p-P70s6K, 4EBP1, p-4EBP1 和 b-actin的1%脱脂牛奶PBS-T里培育2小时.
类似的实验我很久以前做过,很多年不碰生化实验了.你这个实验应该是western blotting最后的几步吧.

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