英语翻译Di- and trimethylation of histone H3 lysine 4 (H3K4me2 and H3K4me3) are hallmarks of chromatin at active genes [1].The major fraction of K4-methylated histone H3 is the variant H3 (termed H3.3 in Drosophila) [2],which replaces canonical H

英语翻译Di- and trimethylation of histone H3 lysine 4 (H3K4me2 and H3K4me3) are hallmarks of chromatin at active genes [1].The major fraction of K4-methylated histone H3 is the variant H3 (termed H3.3 in Drosophila) [2],which replaces canonical H
英语翻译
Di- and trimethylation of histone H3 lysine 4 (H3K4me2 and H3K4me3) are hallmarks of chromatin at active genes [1].The major fraction of K4-methylated histone H3 is the variant H3 (termed H3.3 in Drosophila) [2],which replaces canonical H3 (H3.2) in transcribed genes [3,4].Here,we genetically address the invivo significance of such K4 methylation by replacing wild-type H3.3 with a mutant form (H3.3K4A) that cannot be methylated.We monitored the transcription that occurs in response to multiple well-described signaling pathways.Surprisingly,the transcriptional outputs of these pathways remain intact in H3.3K4A mutant cells.Even the complete absence of both H3.3 genes does not noticeably affect viability or function of cells:double mutant animals are viable but sterile.Fertility can be rescued by K4-containing versions of H3.3,but not with mutant H3.3 (H3.3K4A) or with canonical H3.2.Together,these data suggest that in Drosophila,presence of H3.3K4me in the chromatin of active genes is dispensable for successful transcription in most cells and only plays an important role in reproductive tissues.

英语翻译Di- and trimethylation of histone H3 lysine 4 (H3K4me2 and H3K4me3) are hallmarks of chromatin at active genes [1].The major fraction of K4-methylated histone H3 is the variant H3 (termed H3.3 in Drosophila) [2],which replaces canonical H
见dis -和trimethylation H3的H3K4me2 4组,H3K4me3赖氨酸)的活性基因染色质[1].最主要的部分是K4-methylated组H3变种(称为H3.3 H3的果蝇)[2],代替正则H3(H3.2)的基因(3,4]转录.在这里,我们invivo意义的基因地址等四甲基取代野生H3.3与变异形式(H3.3K4A)是不能的甲基化.我们测试了这个转录发生在应对多元工艺信号通路.令人惊讶的是,这些通路的转录输出的H3.3K4A突变细胞.甚至完全没有H3.3基因的影响不明显的能力或功能:动物细胞突变是可行的,但不能生育.可以救K4-containing生育力的版本,而不是用H3.3突变H3.3(H3.3K4A)或以规范H3.2.这些数据表明,在果蝇、存在的H3.3K4me活跃的基因在染色体则可有可无的成功,只有在大多数细胞转录起着至关重要的作用,在生殖器官.
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