英语翻译The nuclear protein NIPP1 (nuclear inhibitor of pro-tein Ser/Thr phosphatase-1) interacts with the splicingfactors SAP155 and CDC5L and is involved in a late stepof spliceosome assembly.In addition,NIPP1 is an inter-actor of protein phosp

英语翻译The nuclear protein NIPP1 (nuclear inhibitor of pro-tein Ser/Thr phosphatase-1) interacts with the splicingfactors SAP155 and CDC5L and is involved in a late stepof spliceosome assembly.In addition,NIPP1 is an inter-actor of protein phosp
英语翻译
The nuclear protein NIPP1 (nuclear inhibitor of pro-
tein Ser/Thr phosphatase-1) interacts with the splicing
factors SAP155 and CDC5L and is involved in a late step
of spliceosome assembly.In addition,NIPP1 is an inter-
actor of protein phosphatase-1 and a COOH-terminal
NIPP1 fragment displays an RNase E like endoribo-
nuclease activity.A yeast two-hybrid screening resulted
in the identification of the Polycomb group protein EED
(embryonic ectoderm development),an established
transcriptional repressor,as a novel NIPP1 interactor.
NIPP1 only interacted with full-length EED,whereas
two EED interaction domains were mapped to the cen-
tral and COOH-terminal thirds of NIPP1.The NIPP1-
EED interaction was potentiated by the binding of (d)G-
rich nucleic acids to the central domain of NIPP1.
Nucleic acids also decreased the potency of NIPP1 as an
inhibitor of PP1,but they did not prevent the formation
of a ternary NIPP1 EED PP1 complex.EED had no effect
on the function of NIPP1 as a splicing factor or as an
endoribonuclease.However,similar to EED,NIPP1
acted as a transcriptional repressor of targeted genes
and this NIPP1 effect was mediated by the EED interac-
tion domain.Also,the histone deacetylase 2 was present
in a complex with NIPP1.Our data are in accordance
with a role for NIPP1 as a DNA-targeting protein for
EED and associated chromatin-modifying enzymes.
要求大致意思正确,通顺能懂 不要直接用软件翻译的

英语翻译The nuclear protein NIPP1 (nuclear inhibitor of pro-tein Ser/Thr phosphatase-1) interacts with the splicingfactors SAP155 and CDC5L and is involved in a late stepof spliceosome assembly.In addition,NIPP1 is an inter-actor of protein phosp
NIPP1核蛋白(丝氨酸/苏氨酸蛋白磷酸酶-1抑制剂）与SAP155和CDC5L剪接因子相互作用,并参与进一步剪接体的装配.而且,NIPP1核蛋白是蛋白质磷酸酶-1和NIPP1羧基端片段（该片段能表现出RNA酶E,类似核酸内切酶的活性）的连接体.
酵母的双向杂交试验增加了我们对聚合组蛋白EED的认识,EED是一种已建立的转录抑制因子,它是作为一种新型的NIPP1连接体.
NIPP1只与全长（完整）的EED相互作用,然而,与EDD的相互作用被定位在NIPP1的中央和三分之二处.
NIPP1-EED相互作用在富含鸟嘌呤核苷酸之中央的区域被加强.
核酸也可以作为一种PP1抑制剂削弱NIPP1的活性,但是,他们不会阻止NIPP1-EED-PP1三元复合体的形成.
EED作为一种剪接体或者核酸内切酶对NIPP1的功能没有影响.
然而,就像EED,NIPP1充当一种抑制靶向基因转录作,NIPP1的效应被EED的相互作用域调控.
同样,组蛋白2是与NIPP1形成的复合体.
我们的数据是根据NIPP1作为一种通过EED连接的DNA靶蛋白,我们的数据也和核染色质修饰相关酶进行了联系.
抱歉,有些术语我也没听说过,可能翻译的较为生硬,希望您结合整个文献参考吧.

核蛋白NIPP1(蛋白丝氨酸/苏氨酸磷酸酶1的核抑制剂)可与剪接因子SAP155和CDC5L相互作用，并参与剪接体的组装的最后一步。此外，NIPP1是一种能与蛋白磷酸酶1互作的蛋白，它的羧基端（COOH-端）端片断有 RNase E类似的内切核糖核酸酶活性。Polycomb类蛋白EED（胚胎外胚层发育），是已知的一种转录阻遏物，被认为是一个新的NIPP1互作蛋白，对其进行酵母双杂交筛选实验。结果表...

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核蛋白NIPP1(蛋白丝氨酸/苏氨酸磷酸酶1的核抑制剂)可与剪接因子SAP155和CDC5L相互作用，并参与剪接体的组装的最后一步。此外，NIPP1是一种能与蛋白磷酸酶1互作的蛋白，它的羧基端（COOH-端）端片断有 RNase E类似的内切核糖核酸酶活性。Polycomb类蛋白EED（胚胎外胚层发育），是已知的一种转录阻遏物，被认为是一个新的NIPP1互作蛋白，对其进行酵母双杂交筛选实验。结果表明：NIPP1只能与全长的EED相互作用，而两个EED互作结构域被定位在NIPP1蛋白的中间及碳端的1/3蛋白长度区。 NIPP1-EED 互作可通过结合富含 (d)G的核酸到 NIPP1蛋白的中央而加强。
核酸可以降低NIPP1作为一个PP1抑制剂的效力，但是它不能阻止NIPP1-EED-PP1 三聚复合体的形成。 NIPP1 作为一个剪接因子或是一个内切核糖核酸酶时，EED对其没有任何的影响。但是，类似于EED，NIPP1作为一个靶基因的转录阻遏物时， NIPP1 效应是需要EED互作结构域介导的。此外，组蛋白去乙酰化酶2存在于这个NIPP1复合体。我们的数据和 NIPP1 是一种作用EED及相关的染色质修饰酶的DNA靶向蛋白是一致的。

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