英语翻译Genetic screen for intercellular gene transfer.(A) Maps of the plastid genome in Pt-spec:gfp plants and the transgenic locus in Nuc-kan:yfp plants.PpsbA and TpsbA,promoter and terminator from the plastid psbA gene; Prrn,promoter from the

英语翻译Genetic screen for intercellular gene transfer.(A) Maps of the plastid genome in Pt-spec:gfp plants and the transgenic locus in Nuc-kan:yfp plants.PpsbA and TpsbA,promoter and terminator from the plastid psbA gene; Prrn,promoter from the
英语翻译
Genetic screen for intercellular gene transfer.
(A) Maps of the plastid genome in Pt-spec:gfp plants and the transgenic locus in Nuc-kan:yfp plants.PpsbA and TpsbA,promoter and terminator from the plastid psbA gene; Prrn,promoter from the plastid rRNA operon; Trps16,terminator from the plastid rps16 gene; Pnos and Tnos,promoter and terminator from the nopaline synthase gene from Agrobacterium tumefaciens; P35S and T35S,promoter and terminator from the cauliflower mosaic virus 35S transcript; LB and RB,left and right borders of the T-DNA region; Eco RV and Xho I,restriction sites used for restriction fragment length polymorphism analysis (fig.S3).
(B) Selection experiments.The grafted stem region was either sectioned (horizontal lines) or directly exposed to selection (bracket).The middle panel shows the arrangement of tissue explants,the right panel a selection plate (right half,stem sections from the graft site; upper left quarter,three stem sections and three leaf explants from Nuc-kan:yfp; lower left quarter,the corresponding explants from Pt-spec:gfp).After 4 weeks on medium with spectinomycin and kanamycin,some explants from the graft site developed growing callus tissue or regenerating shoots (arrows).
(C) Expression and subcellular localization of the fluorescent reporters.The wild type,the two grafting partners,and a YG line were assayed for GFP,chlorophyll (Chl),and YFP fluorescence.

英语翻译Genetic screen for intercellular gene transfer.(A) Maps of the plastid genome in Pt-spec:gfp plants and the transgenic locus in Nuc-kan:yfp plants.PpsbA and TpsbA,promoter and terminator from the plastid psbA gene; Prrn,promoter from the
细胞间基因转移的遗传学检测
A）转基因Pt-spec:gfp 植株的质粒基因组图及Nuc-kan:yfp 植株的转基因插入的基因座位图.
PpsbA 和TpsbA 分别表示来自质体psbA基因的启动子和终止子；Prrn：质体 rRNA 操纵子启动子；Trps16：质体rps16基因的终止子；Pnos 和 Tnos：农杆菌胭脂碱合酶基因的启动子和终止子；P35S 和 T35S：烟草花叶病毒35S转录本的启动子和终止子；LB 和 RB：T-DNA 区的左右边界；Eco RV 和 Xho I：用于限制性片断长度多态性分析的限制性酶切位点（图附3）.
B）筛选试验.移植的茎区以片断（水平排列）或直接筛选（插入培养基?）.中间板显示外植体的排布；右板是一筛选板（右半边：来自移植位点的茎段；左上：来自Nuc-kan:yfp植株的三个茎段和三个叶片外植体；左下：来自Pt-spec:gfp植株相应的外植体）.在含壮观霉素和卡那霉素的培养基中筛选四周,来自移植位点的一些外植体开始长出愈伤组织或再生芽（箭头所示）.
C）荧光报告基因的表达和亚细胞定位.对野生型植株、两个移植的外植体及YG系植株进行GFP荧光、叶绿素荧光和YFP荧光分析