英语翻译The scheme for our analysis is shown infig.S1.Nucleoli were microsurgically removedfrom fully grown oocytes before gonadotropicstimulations in pigs and mice (Fig.1,A and B,fig.S2,and movies S1 and S2) (8).The oocytesfrom which we aspirate

英语翻译The scheme for our analysis is shown infig.S1.Nucleoli were microsurgically removedfrom fully grown oocytes before gonadotropicstimulations in pigs and mice (Fig.1,A and B,fig.S2,and movies S1 and S2) (8).The oocytesfrom which we aspirate
英语翻译
The scheme for our analysis is shown in
fig.S1.Nucleoli were microsurgically removed
from fully grown oocytes before gonadotropic
stimulations in pigs and mice (Fig.1,A and B,
fig.S2,and movies S1 and S2) (8).The oocytes
from which we aspirated a small amount of
nucleoplasm served as controls [fig.S1,shamoperated
(Sham)].The success of enucleolation was
checked immunocytochemically (Fig.1C and fig.S3A).In sham-operated oocytes,the immunofluorescence
against H3K9triMet (trimethylated
histone H3 at Lys9),C23 (nucleolin),and NPM2
(nucleoplasmin 2),as well as the staining
against chromatin by DAPI (4´,6´-diamidino-
2-phenylindole) and Hoechst (bisbenzimide
H33342),demonstrated a single nucleolus surrounded
by a heterochromatin rim (Fig.1C,
Chromatin,and fig.S3A,Hoechst).With this
approach,no nucleoli were detected in enucleolated
oocytes.After the enucleolation,the
germinal vesicle envelope became sealed,as
confirmed by labeling against lamin A/C (nuclear
lamin A and lamin C) (Fig.1C and fig.
S3B,Lamin A/C),and the chromatin remained
inside the germinal vesicle and was not detectable
around isolated nucleoli (Fig.1C,Chromatin,
and fig.S3A,Hoechst).Maturation of
enucleolated oocytes appeared normal,with regular
spindle formation and subsequent polar
body extrusion,and progressed in a similar time
course as in controls to metaphase II at 52%
(93/180) in the pig (fig.S3,D and F) and 90%
(533/592) in the mouse (fig.S3B).Karyotyping
of mouse oocytes at metaphase II revealed
a normal haploid set of chromosomes in
both sham-operated and enucleolated oocytes
(n = 20 chromosomes,fig.S3C).Analysis of
the kinetics of activities of meiotic cell cycle
molecules cyclin-dependent kinase 1 (CDK1) and
mitogen-activated protein (MAP) kinase demonstrates
that these kinase activities fluctuated
similarly in the enucleolated and control pig oocytes
(fig.S3E).These results suggest that oocyte
nucleoli do not contain the essential factor(s) for
oocyte maturation.
In both species,enucleolated and mature
oocytes were efficiently activated parthenogenetically
(pig:100%,212/212; mouse:75%,
157/208) or by fertilization (pig:57%,93/164;
mouse:69%,129/186).Whereas round prominent
nucleoli were detectable in germinal
vesicles and in both the male and female
pronuclei of control zygotes,they were not
visible in germinal vesicles or pronuclei originating
from enucleolated oocytes (Fig.1,D
to F).After activation,CDK1 and MAP kinase
in enucleolated oocytes were inactivated in a
similar time course as in controls (fig.S3E).
At the pronucleus or four- to eight-cell stage,the
proteins and DNA were synthesized at similar
levels in both the experimental and control groups
(fig.S4).

英语翻译The scheme for our analysis is shown infig.S1.Nucleoli were microsurgically removedfrom fully grown oocytes before gonadotropicstimulations in pigs and mice (Fig.1,A and B,fig.S2,and movies S1 and S2) (8).The oocytesfrom which we aspirate
我们的分析图如下显示,图S1:在猪和鼠受到gonadotropic刺激前,Nucleoli已经从完全成熟的oocytes中用超微手术剔除下来了.不翻译了.楼主学生物细胞学啊.

好多啊…在翻译之前，能否联系一下你？