帮忙设计个引物>gi|21407256|gb|AY088482.1| Arabidopsis thaliana clone 7101 mRNA,complete sequenceATTTTCCACAACTTGGAAATCGAAATCCATTTCGAATTTTGGTTAGCAGCGATTTCAAATTCCAAACGTAAGAATTATGGCGGGAGTGAATCAGGCGTGTATCTTCTGTGAGATCGTGCGAAATCCAACCACTACTCGTCTCCTTCA
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帮忙设计个引物>gi|21407256|gb|AY088482.1| Arabidopsis thaliana clone 7101 mRNA,complete sequenceATTTTCCACAACTTGGAAATCGAAATCCATTTCGAATTTTGGTTAGCAGCGATTTCAAATTCCAAACGTAAGAATTATGGCGGGAGTGAATCAGGCGTGTATCTTCTGTGAGATCGTGCGAAATCCAACCACTACTCGTCTCCTTCA
帮忙设计个引物
>gi|21407256|gb|AY088482.1| Arabidopsis thaliana clone 7101 mRNA,complete sequence
ATTTTCCACAACTTGGAAATCGAAATCCATTTCGAATTTTGGTTAGCAGCGATTTCAAATTCCAAACGTA
AGAATTATGGCGGGAGTGAATCAGGCGTGTATCTTCTGTGAGATCGTGCGAAATCCAACCACTACTCGTC
TCCTTCACACCGATGAGAAAGTCATCGCCTTTCAAGACATCAAGCCTGCAGCCCAGAGGCACTATTTGGT
GATTCCAAAAGAACATATTCCTACTGTGAATGACCTTCAGAGAAGAGATGAAGACTACTCACTTGTAAGA
CACATGCTTAGTGTGGGACAACAACTGTTGCAGAAAGATGCTCCTCAAAGCATTCATAGATTTGGTTTTC
ACCAGCCACCATTTAACAGTGTTGATCATCTCCATCTCCACTGTTTTGCATTGCCTTATGTGCCCAGATG
GAAAGCCATCAAGTACAAGTCTTTGGGACCTTTGGGTGGATTTATTGAAGCCGAGACACTGCTAGAGAAG
ATAAGGCCTCTTCTTTCAAAGGTGTMACTGAACCCAACTTGTTTAACCGCATTGCGTTGCTTCTCTTTGC
TAAAACTGTGCAATATCTCTTTTCTATTCTTTTGCTGGCAAATCTGTGAACAAATGCCAAATATTATTAG
AGATTTTGTATATGAAAGAAGGATGTGTCAAGTAATTATATGTAATTTCTGCCATTGTCCAATAGTTAAC
ACTTAACACCAATCTCATTGTTTCCATGCTT
全长CDNA的引物,列出正向引物和反向引物,以及引物长度
帮忙设计个引物>gi|21407256|gb|AY088482.1| Arabidopsis thaliana clone 7101 mRNA,complete sequenceATTTTCCACAACTTGGAAATCGAAATCCATTTCGAATTTTGGTTAGCAGCGATTTCAAATTCCAAACGTAAGAATTATGGCGGGAGTGAATCAGGCGTGTATCTTCTGTGAGATCGTGCGAAATCCAACCACTACTCGTCTCCTTCA
Sequence (5'->3') Strand on template Length Tm GC%
Forward primer CCATTTCGAATTTTGGTTAGCAGCG Plus 25 56.10 44.00%
Reverse primer AGCATGGAAACAATGAGATTGGTGT Minus 25 55.47 40.00%
Product length 704