英语翻译Rapid and sensitive step gradient assays of glutamate,glycine,taurine and g-aminobutyric acid by high-performance liquidchromatography–fluorescence detection with o-phthalaldehyde–mercaptoethanol derivatization with an emphasis on mic

英语翻译Rapid and sensitive step gradient assays of glutamate,glycine,taurine and g-aminobutyric acid by high-performance liquidchromatography–fluorescence detection with o-phthalaldehyde–mercaptoethanol derivatization with an emphasis on mic
英语翻译
Rapid and sensitive step gradient assays of glutamate,glycine,
taurine and g-aminobutyric acid by high-performance liquid
chromatography–fluorescence detection with o-phthalaldehyde–
mercaptoethanol derivatization with an emphasis on microdialysis
samples
T.Petteri Piepponena ,*,Arnis Skujinsa,b
aDepartment of Pharmacy,Division of Pharmacology and Toxicology,P.O.Box 56,University of Helsinki,FIN-00014 Helsinki,
Finland
bLaboratory of Pharmacology,Latvian Institute of Organic Synthesis,21 Aizkraukles Street,Riga LV-1006,Latvia
Received 30 November 2000; received in revised form 26 February 2001; accepted 1 March 2001
Abstract
We developed a rapid step-gradient HPLC method for determination of glutamate,glycine and taurine,and a separate
method for determination of g-aminobutyric acid (GABA) in striatal microdialysates.The amino acids were pre-column
derivatized with o-phthalaldehyde–2-mercaptoethanol by using an automated refrigerated autoinjector.Separation of the
amino acids was established with a non-porous ODS-II HPLC column,late-eluting substances were washed out with a
one-step low-pressure gradient.Concentrations of the amino acids were determined with a fixed-wavelength fluorescence
detector.The detection limit for GABA was 80 fmol in a 15 ml sample,detection limits for glutamate,glycine and taurine
were not determined because their concentrations in striatal perfusates were far above their detection limits.Total analysis
time was less than 12 min,including the wash-out step.The methods described are relatively simple,sensitive,inexpensive,
and fast enough to keep up with the microdialysis sampling.Ó 2001 Elsevier Science B.V.All rights reserved.
Keywords:Derivatization,LC; Glutamate; Glycine; Taurine; g-Aminobutyric acid

英语翻译Rapid and sensitive step gradient assays of glutamate,glycine,taurine and g-aminobutyric acid by high-performance liquidchromatography–fluorescence detection with o-phthalaldehyde–mercaptoethanol derivatization with an emphasis on mic
Rapid and sensitive step gradient assays of glutamate,glycine,
快速、敏感的谷氨酸盐步梯度检测,甘氨酸,
taurine and g-aminobutyric acid by high-performance liquid
富含牛磺酸及g-aminobutyric酸的高效液相
chromatography–fluorescence detection with o-phthalaldehyde–
chromatography-fluorescence检测与o-phthalaldehyde -
mercaptoethanol derivatization with an emphasis on microdialysis
mercaptoethanol derivatization强调微量透析可把时程延长
samples
样品
T.Petteri Piepponena ,*,Arnis Skujinsa,b
t .Petteri Piepponena、*、Arnis Skujinsa,b
aDepartment of Pharmacy,Division of Pharmacology and Toxicology,P.O.Box 56,University of Helsinki,FIN-00014 Helsinki,
aDepartment药房的分割药理学和毒理学、邮政信箱56个,赫尔辛基大学、鳍- 00014年赫尔辛基,
Finland
芬兰
bLaboratory of Pharmacology,Latvian Institute of Organic Synthesis,21 Aizkraukles Street,Riga LV-1006,Latvia
bLaboratory药理学,拉脱维亚研究所的有机合成、21 Aizkraukles街、里加LV - 1006、拉脱维亚
Received 30 November 2000; received in revised form 26 February 2001; accepted 1 March 2001
2000年11月30日收到;收到来自26修订2001年2月,接受了2001年3月1日
Abstract
文摘
We developed a rapid step-gradient HPLC method for determination of glutamate,glycine and taurine,and a separate
我们开发了一个快速step-gradient反相高效液相色谱法测定麸胺酸、甘氨酸、牛磺酸、和一个单独的
method for determination of g-aminobutyric acid (GABA) in striatal microdialysates.The amino acids were pre-column
方法测定g-aminobutyric酸(GABA)microdialysates纹状体.pre-column的氨基酸
derivatized with o-phthalaldehyde–2-mercaptoethanol by using an automated refrigerated autoinjector.Separation of the
与o-phthalaldehyde-2-mercaptoethanol表现出特征性利用自动化冷藏自动注射剂.分离的
amino acids was established with a non-porous ODS-II HPLC column,late-eluting substances were washed out with a
氨基酸是建立了一个干燥的物体ODS-II高效液相色谱柱、late-eluting物质被淘汰的用
one-step low-pressure gradient.Concentrations of the amino acids were determined with a fixed-wavelength fluorescence
一步低压梯度.氨基酸的含量测定fixed-wavelength荧光
detector.The detection limit for GABA was 80 fmol in a 15 ml sample,detection limits for glutamate,glycine and taurine
探测器.GABA的检测限度是80 fmol 15毫升样品,检测限度麸胺酸、甘氨酸、牛磺酸
were not determined because their concentrations in striatal perfusates were far above their detection limits.Total analysis
不确定,因为他们的含量perfusates纹状体是远远高于他们的检测范围.全面分析
time was less than 12 min,including the wash-out step.The methods described are relatively simple,sensitive,inexpensive,
时间少于12分钟,包括本事一步.描述的方法相对简单、敏感、便宜,
and fast enough to keep up with the microdialysis sampling.Ó 2001 Elsevier Science B.V.All rights reserved.
和足够快跟上微量透析可把时程延长采样.2001年阿爱科学.保留所有权利.
Keywords:Derivatization,LC; Glutamate; Glycine; Taurine; g-Aminobutyric acid
关键词:Derivatization、LC、谷氨酸、甘氨酸、牛磺酸、g-Aminobutyric酸