化学文献翻译20分一段~第一段2.3 Physical measurements1H NMR spectra were recorded on an Avance-400 spectrometer with d6-DMSO (dimethyl sulfoxide) as the solvent at room temperature and TMS (tetramethylsilane) as the internal standard. UV

化学文献翻译20分一段~第一段2.3 Physical measurements1H NMR spectra were recorded on an Avance-400 spectrometer with d6-DMSO (dimethyl sulfoxide) as the solvent at room temperature and TMS (tetramethylsilane) as the internal standard. UV
化学文献翻译20分一段~第一段
2.3 Physical measurements
1H NMR spectra were recorded on an Avance-400 spectrometer with d6-DMSO (dimethyl sulfoxide) as the solvent at room temperature and TMS (tetramethylsilane) as the internal standard. UV–Vis (UV–visible) spectra were recorded on a Perkin–Elmer Lambda-25 spectrophotometer, and emission spectra were recorded on a PerkinElmer LS-55 luminescence spectrometer at room temperature. Electrospray mass spectra (ES-MS) were recorded on a LQC system (Finngan MAT, USA) using CH3CN as the mobile phase.
The absorption titration of ruthenium(II) complexes in buffer was carried out by adding the DNA stock solution to a fixed concentration of ruthenium. Ruthenium solutions employed were 10 μM in concentration and calf thymus DNA was added to a ratio of 1.27:1 [DNA]/[Ru]. Ruthenium-DNA solutions were allowed to incubate for 5 min before the absorption spectra were recorded. The intrinsic binding constants Kb of Ru (II) complexes to DNA were calculated from equation [14]:
[DNA] / (εa -f) = [DNA] / (b -f) + 1 / [Kb(b -f)]
Where [DNA] is the concentration of DNA in base pairs, εa, εf and εb correspond to the apparent absorption coefficient Aobsd/[Ru], the extinction coefficient for the ruthenium complex in the fully bound form, respectively. In plots of [DNA]/( εa -εf ) versus [DNA], Kb is given by the ratio of slope to the intercept.
Viscosity measurements were carried out by using an Ubbelodhe viscometer maintained a constant temperature at 28.0 (±0.1) C in thermostatic water-bath. CT-DNA samples approximately 200 base pairs in average length were prepared by sonicating in order to minimize complexities arising from DNA flexibility [15]. Flow time was measured with a digital stopwatch, and each sample was measured three times, and an average flow time was calculated. Data were presented as (/0) 1/3 versus binding ratio [16], where  is the viscosity of CT-DNA in the presence of complex, and 0 is the viscosity of CT-DNA alone.

化学文献翻译20分一段~第一段2.3 Physical measurements1H NMR spectra were recorded on an Avance-400 spectrometer with d6-DMSO (dimethyl sulfoxide) as the solvent at room temperature and TMS (tetramethylsilane) as the internal standard. UV
2.3物理测量
核磁共振谱记录的进步 - 400谱仪与D6中-二甲基亚砜（二甲亚砜）为溶剂,在室温和全方位维修计划作为内部标准.紫外可见光谱（紫外可见）谱记录的一珀金-埃尔默的LAMBDA - 25分光光度计,和发射光谱均录得就一珀金埃尔默的LS - 55发光光谱仪在室温下.电喷雾质谱（安老服务- MS ）的均录得就一lqc系统（ finngan垫,美国）使用乙腈作为流动相.
吸收滴定法测定钌（二）配合在缓冲区进行了加入的DNA原液,以一个固定的浓度钌.钌解决方案,聘请10 μ m的浓度和小牛胸腺DNA ,加入的比例为1.27:1 [ DNA的] / [如] .钌- DNA的解决方案,获准以孵化为5分钟前,吸收光谱均录得.内在的结合常数KB的茹（二）配合物的DNA计算从方程[ 14 ] ：
[ DNA的] / （ ε 1 -六） = [ D NA的] / （  B组- 六） + 1 / [ K B （下 B组-  f）项]
其中[ DNA的]是浓度的DNA的碱基对, ε 1 , ε f和ε b对应的表观吸收系数aobsd / [如] ,消光系数为钌配合物在完全约束的形式,分别为.在图谋[ DNA的] / （ ε 1 -ε f ）在银两[ D NA的] ,或给出的比例斜坡向拦截.
粘度测量所进行的使用乌伯娄德粘度计粘度保持温度恒定在28.0 （ ± 0.1 ）  C在恒温水浴.电脑断层的DNA样本约200个碱基对在平均长度分别编写的sonicating ,以尽量减少复杂性所引起的DNA的灵活性, [ 15 ] .流动时间是衡量一台数码秒表,每个样品测量的3倍,平均流通时间计算.数据作为（  /  0 ） 1 / 3银两约束力的比率[ 16 ] ,其中是粘度的CT - DNA的存在复杂,  0是粘度的CT - DNA的单.

.3物理测量
1H核磁共振光在与d6-DMSO (二甲亚砜)的一台Avance-400分光仪被记录了作为在室温和TMS (tetramethylsilane)的溶剂作为内部标准。 UV–Vis (UV–visible)光谱在Perkin–Elmer Lambda25分光光度表被记录了，并且发射光谱在室温的一台PerkinElmer LS-55发光学分光仪被记录了。 Electrospra...

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.3物理测量
1H核磁共振光在与d6-DMSO (二甲亚砜)的一台Avance-400分光仪被记录了作为在室温和TMS (tetramethylsilane)的溶剂作为内部标准。 UV–Vis (UV–visible)光谱在Perkin–Elmer Lambda25分光光度表被记录了，并且发射光谱在室温的一台PerkinElmer LS-55发光学分光仪被记录了。 Electrospray质谱(ES-MS)在LQC系统(Finngan席子，美国)被记录了使用CH3CN作为流动相。
The钌的吸收滴定法(II)在缓冲的复合体通过增加脱氧核糖核酸储备溶液执行到钌的固定的集中。 钌解答使用是10 μM在集中，并且小牛胸腺脱氧核糖核酸是增加对比率1.27:1 [脱氧核糖核酸]/[Ru]。 5分钟，在吸收光谱被记录了之前，钌脱氧核糖核酸解答允许孵化为。 内在约束常数千字节对脱氧核糖核酸的Ru (ii)复合体从式[14]被计算了：
[脱氧核糖核酸]/(εa - f) = [脱氧核糖核酸]/(b - f) + 1/[千字节(b - f)]
Where [脱氧核糖核酸]分别为脱氧核糖核酸的集中在基本的对的， εa、εf和εb对应于明显的吸收系数Aobsd/[Ru]，钌复合体的消光系数以充分地束缚型。 在剧情[脱氧核糖核酸]/(εa - εf)对[脱氧核糖核酸]，千字节由倾斜比给与截住。
Viscosity测量被执行了通过使用Ubbelodhe黏度计维护了恒温在恒温热水锅的28.0 (±0.1) C。 CT-DNA抽样大约200个基本的对平均长通过为了使复杂减到最小的声波处理准备出现从脱氧核糖核酸灵活性[15]。 流程时间测量了与一块数字式秒表，并且每个样品被测量了三次，并且平均流程时光被计算了。 提出了数据(/0) 1/3对约束比率[16]，其中是CT-DNA黏度在复合体面前和0单独CT-DNA黏度。

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