英语翻译Infection by Enterobacter sakazakii has emerged as a rare cause of neonatal meningitis,septicemia,and Enterocolitis.Contaminated infant formula has been the principle infection or transmission vehicle,although E.sakazakii has been isolate

英语翻译Infection by Enterobacter sakazakii has emerged as a rare cause of neonatal meningitis,septicemia,and Enterocolitis.Contaminated infant formula has been the principle infection or transmission vehicle,although E.sakazakii has been isolate
英语翻译
Infection by Enterobacter sakazakii has emerged as a rare cause of neonatal meningitis,septicemia,and Enterocolitis.Contaminated infant formula has been the principle infection or transmission vehicle,although E.sakazakii has been isolated from a wide range of foods,food ingredients or food production environments.To date,the true reservoir and the exact epidemiology are still unknown.Although not all cased have been attributed to the ingestion of infant formula (IFM),the microbiological safety and preparation of IFM are of great concern.The international commission for Microbiological Specification for Foods has ranked E.sakazakii as a “Severe hazard for restricted populations,life
Threatening or substantial chronic sequelae or long duration”.To minimize the possible contamination of IFM,both the raw material and the production environment must be constantly monitored.In general,microbiological methods based on phenotype anlysis are acknowledeged to be unreliable due to the unstable expression of corresponding marker(s).In 2004,the joint FAO/WHO workshop on E.sakazakii and other micoorganisms in powdered infant formula recommended promoting the use of internationally validated detection and molecular typing methods for identifying E.sakazakii.
In this contest,methods employing molecular subtypings based on DNA,such as RAPD-PCR,can be useful tools to facilitate surveillance,and can also trace routes from a source to an infected individual.To date,however,there has been only one report on the use of ERIC-PCR fingerprinting in E.sakazakii ATCC51329.The aim of the current study was therefore to demonstrate the potential usefulness of the ERIC-PCR technique in the investigation of epidemic disease,as well as for basic molecular characterizations of pathogenic organisms such as E.sakazakii.ERIC-PCR subtyping fingerprinting was used to generate fingerprint patterns from the type strain ATCC29544 as well as from 22 E.sakazakii strains derived from infant formular and powdered milks.One consensus fragment was found in all of the ERIC-PCR fingerprints.This fragment was cloned and sequenced from the type strain ATCC29544 and dot hybridization confirmed its presence in all of the other 22strains.The sequence of this consensus fragment was then used to examine diversity within E.sakazakii strains and other related species.

英语翻译Infection by Enterobacter sakazakii has emerged as a rare cause of neonatal meningitis,septicemia,and Enterocolitis.Contaminated infant formula has been the principle infection or transmission vehicle,although E.sakazakii has been isolate
我翻得不是很完美,如有更好的答案,那就采纳他们的吧.
由阪崎肠杆菌感染已成为一个新生儿脑膜炎,败血症罕见的原因,肠炎.受污染的婴儿配方原则一直是感染或传播手段,但阪崎肠杆菌,已由一个范围广泛的食品隔离,食品或食品配料生产环境.迄今为止,真正的水库和准确的流行病学还是一个未知数.虽然并非所有套管已归因于婴儿配方（摄入的IFM）,微生物安全,易福门的准备非常关注.为规范食品微生物国际委员会已列为一个“限制人口,严重危害生命阪崎肠杆菌
威胁或大量或长期慢性后遗症“.为了最大限度地减少污染的IFM可能,无论是原材料和生产环境必须不断进行监测.一般而言,微生物学方法对表型的基础理论视角下的acknowledeged是不可靠的,由于相应的标记（第不稳定）的表达.2004年,联合粮农组织/世界卫生组织关于阪崎肠杆菌和其他婴儿配方奶粉micoorganisms讲习班建议推广使用国际认可的检测和分子分型鉴定阪崎肠杆菌的方法.
在这场竞争中,采用的方法,如分子subtypings的RAPD - PCR技术对DNA的基础上,可以成为有用的工具,以方便监视,跟踪,还可以从一个源路由到一个受感染的个人.迄今为止,然而,至今只有一对ERIC - PCR技术在阪崎肠杆菌ATCC51329指纹使用情况的报告.当前研究的目的,因此证明,如阪崎肠杆菌的流行性疾病的调查李家祥- PCR技术,以及病原微生物的基本分子刻画潜在的用处.ERIC - PCR技术分型指纹是用来产生从类型菌株ATCC29544指纹图谱,以及从22 e.从婴儿配方奶粉和牛奶派生阪崎肠杆菌菌株.一个共识片段被发现的ERIC - PCR技术指纹的.这片段的克隆和测序型菌株ATCC29544和斑点杂交证实在其他22strains所有它的存在.在这个共识的片段序列,然后用阪崎肠杆菌菌株内审查和其他相关的物种多样性.