冻干粉蛋白质试剂如何分装?我买的是sigma公司的血管紧张素Ⅱ冻干粉1mg,我每次用量很小,向分装以下,应该怎么办?怎么样算是适量？我又不想称量损失试剂，因为只有1mg啊
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冻干粉蛋白质试剂如何分装?我买的是sigma公司的血管紧张素Ⅱ冻干粉1mg,我每次用量很小,向分装以下,应该怎么办?怎么样算是适量？我又不想称量损失试剂，因为只有1mg啊
冻干粉蛋白质试剂如何分装?
我买的是sigma公司的血管紧张素Ⅱ冻干粉1mg,我每次用量很小,向分装以下,应该怎么办?
怎么样算是适量？我又不想称量损失试剂，因为只有1mg啊
冻干粉蛋白质试剂如何分装?我买的是sigma公司的血管紧张素Ⅱ冻干粉1mg,我每次用量很小,向分装以下,应该怎么办?怎么样算是适量？我又不想称量损失试剂，因为只有1mg啊
楼主如果订的是Angiotensin II human 的话你可以看下sigma的datasheet
Storage/Stability
It is recommended to store angiotensin II at –20 C.
Lyophilized peptides are generally extraordinarily stable
at sub-zero temperatures,often showing little or no
degradation even after years.To avoid condensation of
moisture in the bottle,allow the vial to reach room
temperature before opening.If the contents of a vial
are not used immediately,blanket with an inert gas
(nitrogen) and store desiccated in the dark at –20 C.
Stock solutions at neutral pH stored at –20 C should
remain active for at least two months.Avoid repeated
freeze-thaw cycles.The reconstituted peptide solution
may also be aliquoted,relyophilized,and stored
at –20 C.Peptides in solution are also susceptible to
proteolytic degradation.Proteases of microbial origin
may be excluded by the use of freshly distilled water
and sterile filtration.Gloves are recommended to avoid
chance contact with human proteases present on the
skin.
太专业了，不懂
很简单啊。
你取适量冻干粉配成储液，储液浓度一般应该是工作浓度的50倍以上，至少10倍以上。然后由储液稀释成为工作液，将工作液分装到几个micro ep 管里，每次就取一管。就不会造成药品反复冻融的损失了~！
至于你分装的体积，就看你自己的了。我习惯于一管用十次或者五次。...
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很简单啊。
你取适量冻干粉配成储液，储液浓度一般应该是工作浓度的50倍以上，至少10倍以上。然后由储液稀释成为工作液，将工作液分装到几个micro ep 管里，每次就取一管。就不会造成药品反复冻融的损失了~！
至于你分装的体积，就看你自己的了。我习惯于一管用十次或者五次。
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