iPS细胞可在胚胎中正常发育吗?
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iPS细胞可在胚胎中正常发育吗?
iPS细胞可在胚胎中正常发育吗?
iPS细胞可在胚胎中正常发育吗?
iPS细胞可在胚胎中正常发育
To our knowledge,for the first time,we demonstrate that induced pluripotent stem cells (iPSCs) can autonomously generate full-term mice via tetraploid blastocysts complementation.Differentiated somatic cells can be reprogrammed into iPSCs by forced expression of four transcription factorsOct4,Sox2,Klf4,and c-Myc.However,it has been unclear whether reprogrammed iPSCs are fully pluripotent,resembling normal embryonic stem cells (ESCs),as no iPSC lines have shown the ability to autonomously generate full-term mice after injection into tetraploid blastocysts.Here we provide evidence demonstrating that an iPSC line induced by the four transcription factors can be used to generate full-term mice from complemented tetraploid blastocysts and thus appears to be fully pluripotent.This work serves asa proof of principle that iPSCs can in fact generate full-term embryos by tetraploid complementation.