英语翻译ResultsDetection of upregulated proteinsIn order to identify membrane proteins of K562 cells withincreased expression under iron deprivation,we isolatedhydrophobic membrane proteins from the cells after 24-hincubation under control condit

英语翻译ResultsDetection of upregulated proteinsIn order to identify membrane proteins of K562 cells withincreased expression under iron deprivation,we isolatedhydrophobic membrane proteins from the cells after 24-hincubation under control condit
英语翻译
Results
Detection of upregulated proteins
In order to identify membrane proteins of K562 cells with
increased expression under iron deprivation,we isolated
hydrophobic membrane proteins from the cells after 24-h
incubation under control conditions (defined transferrin
medium supplemented with 5 lg/ml of transferrin as the
source of iron) and under iron deprivation (defined ironfree
medium without any added source of iron,see Materials
and methods) using a two-phase partitioning system
(2-D Sample Prep for Membrane Proteins from Pierce,see
Materials and methods).We prepared three pairs of 2D gels
from three separate pairs of samples of control cells and
cells cultured under iron deprivation.Gels were stained
using colloidal Coomassie blue.After scanning the stained
gels in a densitometer,we analyzed triplets of gel images
using PDQuest 7.1 2-D Analysis Software in semimanual
detecting and matching mode.
Approximately 300 stable spots were detected on each gel
map.The overall protein expression profiles with pI 3–11
and molecular mass of 20–120 kDa were very similar within
the three gels of both groups of samples (control and iron
deprivation).After the electronic construction of a virtual
Master gel,we searched for spots representing proteins with
significantly increased expression under iron deprivation
when compared with the expression under control conditions.
The increased expression under iron deprivation was
shown to be statistically significant in the case of two spots,
i.e.,spot 1 (P\0.05) and spot 2 (P\0.01).Positions of
these two spots on representative 2Dgels are shown in Fig.1
and the effect of iron deprivation on the expression of proteins
of these spots is shown in Fig.2.

英语翻译ResultsDetection of upregulated proteinsIn order to identify membrane proteins of K562 cells withincreased expression under iron deprivation,we isolatedhydrophobic membrane proteins from the cells after 24-hincubation under control condit
结果
检测上调的蛋白质
为了确定膜蛋白的K562细胞与
表达增加铁剥夺下,我们孤立
疏水膜蛋白的细胞后24小时
孵化控制条件下（定义转
培养基5 LG电子/毫升的转
来源铁）和铁剥夺（定义ironfree
中期没有任何补充铁的来源,见材料
和方法）使用的是两相分配系统
（ 2三维样品制备的膜蛋白由皮尔斯,见
材料和方法） .我们准备三双二维凝胶
从三个不同的对样品的控制细胞和
细胞培养铁剥夺.凝胶染色
用胶体考马斯亮蓝.扫描后的彩色
凝胶的密度,我们分析了三胞胎的凝胶图像
使用PDQuest 7.1二维分析软件在semimanual
检测和匹配模式.
大约300名稳定的景点发现每个凝胶
地图.总的蛋白质表达谱与电3-11
和分子量20-120 kDa的非常相似的
三胶了这两个群体的样本（控制和铁
剥夺） .在电子建设一个虚拟
硕士凝胶,我们寻找景点代表蛋白
表达显着增加下铁剥夺
相比下的表达控制的条件.
增加表达的是铁剥夺
显示出统计学的情况下两个位置,
即,现货1 （ P \ 0.05 ）和现场2 （ P \ 0.01 ） .职位
这两个景点的代表2Dgels中显示图.1
的影响铁剥夺表达的蛋白质
这些景点是在图所示.2 .