克隆出牛的leptin基因的cdna全长序列?(用race)
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克隆出牛的leptin基因的cdna全长序列?(用race)
克隆出牛的leptin基因的cdna全长序列?(用race)
克隆出牛的leptin基因的cdna全长序列?(用race)
不用做RACE,GenBank有完整序列:
>gi|87196504|ref|NM_173928.2| Bos taurus leptin (LEP),mRNA
CGGCGCCAGCAGCGGCGAGGCCCCAGAAGCCCATCCCGGGAAGGAAAATGCGCTGTGGACCCCTGTATCG
ATTCCTGTGGCTTTGGCCCTATCTGTCTTACGTGGAGGCTGTGCCCATCTGCAAGGTCCAGGATGACACC
AAAACCCTCATCAAGACAATTGTCACCAGGATCAATGACATCTCACACACGCAGTCCGTCTCCTCCAAAC
AGAGGGTCACTGGTTTGGACTTCATCCCTGGGCTCCACCCTCTCCTGAGTTTGTCCAAGATGGACCAGAC
ATTGGCGATCTACCAACAGATCCTCACCAGTCTGCCTTCCAGAAATGTGGTCCAAATATCCAATGACCTG
GAGAACCTCCGGGACCTTCTCCACCTGCTGGCCGCCTCCAAGAGCTGCCCCTTGCCGCAGGTCAGGGCCC
TGGAGAGCTTGGAGAGCTTGGGCGTTGTCCTGGAAGCTTCCCTCTACTCCACCGAGGTGGTGGCCCTGAG
CCGGCTGCAGGGGTCACTACAGGACATGTTGCGGCAGCTGGACCTCAGTCCCGGGTGCTGAAGCCTTGAA
GGCCTCTCTTCCCAAAGTCCAGGGAAGAAACCTGAGCTTCTGGCTGTCCACAGGAGAAGAGAGCCTATGT
GGGCATCCTTTATGCAGGCCAGCGGGCCATTTCTCTCTCGCTCCTCTCAGCTGCTCTTCCAAAGGCAGAA
AACTGCGAGGCAGGAAACCAAAGATATAAATACAGATTCCACGCCCACCGGGAAGGGGGGCCCATCCAGC
AAACACTAGACCGGAGCTGGGATTTTCACAGCAGTCTTCCTCCCTGTTCCAGCTCCCTCTCACTGCATGC
TTCAGCATGACCTGGGGTGATTTCAGAGCCTTTGGACCATCAAGCAAGATTCCCTCTGAGAATCCAGGGA
GCATCATGAAGGCTACAGCACATACAGCTGGATATTCCCACACAACATACGATGGAAGCATTTATTTATT
TATTATGCATTTTATTCTGAATGAATTTGAAGCAAAACACCAGCTCTTCCAGGCTCTTTGGGGTCAGCTG
GGGTGAGGAACGCTCCTGGGGTGCCCATCGACAGGCCTCACTGAGGCAAACCCATTTTGAGTGACTTGAG
GCCTCTCAAGTTTGTTCTCCAGGGACTGGCTTTGTTTCTACTGTGACTGACTTTAAATTACAGTGTTTGC
AATGGCATTGCTCTGAATGGATCTTGAAGGACCAAGTTGTTTTAAAAAGAAGAAGATGAATTTTGTCAAG
TGTAATATATCACTGGGTATAACCCGGAGGTGGGAGATGTGTTGATGGGAGCGGGGGAGATCCAGAATGT
GTTTTCTGAATAACATTTGTGTGATGGACTCTTTGGATGGGGTGAGGTCATCTTCTCATCTTTGTGGTTT
TCATGAAGAGGAGATGACTCCTTCAGGGGATTGTGGGGGTTGGCCAACCATCCATGGATGGAGTGGTGGG
GGCACTGAAGCTGAAGACCGTCGGGGTAGTGGTGAGCTCTGGCCTTCTCTGACTGTTAGAGAGTGGTCTT
GCTCATCAGGAAGTGAGGACCCCACACTGGAGATGGTGACCCCCAGAGCAGGGGTCCTTGGTATGAATGG
TCTGGGTTGACCCCACACTGTGTTGATAACATGGTCGTGACCTTCTTTGGGATTTGCATGCTCACCCAAA
GCAAGGCCATGTTTCCCATCCATTTGGGAAGGATTTTCATTCCAGTGGGAGAGGGAAGTATTCCAGTGTG
GACTTCAGTGGATGGTCCCTCGACCTGGGTCAGCAATGGGTCAGTTGAGGCCCAAGACCCCAGGACCAGC
CCCCAGGAGCCTCCTTCTCACTAGTGGTCATGTGCAGTAGAACAAAAGAGGAGGCTTGGGTTTCCCACCA
ACCTGCCATTGTGATGCAGCCGTCACACAACAGGAGGTGGATCTGTCCAAGGAAATTGGAATCTAAGCAA
CCAATTTTAAGACTGAGCACCTACTTGTGCTCAGCCCCAACTGGTGCTATGGGCTGAGAAGCTCACCAAA
TAAATATTAAAATGCAAGCCCTGCCCTCAGGGACCTTGCATTCCAGATGGCAGAATCCCACTCACCAGCA
TGCAAAGGCTGCCGTTTCACCATGGCAACTGAGCAGCTGAGACAGTGCAGTCCTCAGCAGGTGGGAAATG
CTGAGCTGTGGAGGGCAGTGCCCAGGGGCCTACAGGCTAACTGTGCTTGCACTTGGCAGCAGTTTTACTT
TTCAGGGCACGTCAGCATCTATTACTGTGATGCCACATCCCTTTGAAGCGGGATAGCTAAGAATTTAATA
AGAAGAAAATACCTAAGACCATAACAGCAGACAGGGGGCAGGACCAAGTCTAGAATCCTGGTCCTCTGAC
CTCCAGAGTGCCCCTTGAGCCAGGTGGTAGTCTCTGGAGATGTGAACAAAGTAGGGCAGGGAGGGCAGGA
GTGTTTGCTGGAAGAGAGGAGTTCCGAGGCCAATTTTGCAGGCAGTGAGGGAAGTGAATTGCCTGGAGGG
AGGAGGCTGTTTTGTTTGAAGCTTCGGTCTGAGACACCGAAGGGAGGTGATAGGGTGAGCAGCTAGTTAC
AAAGAAAGGCAGAGGAAGCAAAGATGGGAGGGAAGGGTTATGTTGAAGAGGACTTCAGGGGCCAAGACAT
TTGCTACTGAAAGAGATAAGGGTGAAAAGTTCTAGAACGAGACTCATGTGATGGACAGAGTCAGACCTGC
TCGAGAAGATAAGTCCTGATGATCACAGCCCAGTCAGACCGGGATGTTTTAAGCCTTTTGCTCACAAAAC
CTGGCATGGCACTAGGGCTTGTTCCCAGAGTGTGAAACTTCCAAAATATAAATGATTGTGTTTTTCTGTA
ACTTAAAACAATTTTTTTTTGTTGTTCCAAAAAGTCCAAATAAATGACCTTTGCCCCTTG