英语翻译Powdery mildew fungi are obligate biotrophic pathogens that only grow on living hosts and cause damage in thousands of plant species.Despite their agronomical importance,little direct functional evidence for genes of pathogenicity and vir

英语翻译Powdery mildew fungi are obligate biotrophic pathogens that only grow on living hosts and cause damage in thousands of plant species.Despite their agronomical importance,little direct functional evidence for genes of pathogenicity and vir
英语翻译
Powdery mildew fungi are obligate biotrophic pathogens that only grow on living hosts and cause damage in thousands of plant species.Despite their agronomical importance,little direct functional evidence for genes of pathogenicity and virulence is currently available because mutagenesis and transformation protocols are lacking.Here,we show that the accumulation in barley (Hordeum vulgare) and wheat (Triticum aestivum) of double-stranded or antisense RNA targeting fungal transcripts affects the development of the powdery mildew fungus Blumeria graminis.Proof of concept for host-induced gene silencing was obtained by silencing the effector gene Avra10,which resulted in reduced fungal development in the absence,but not in the presence,of the matching resistance gene Mla10.The fungus could be rescued from the silencing of Avra10 by the transient expression of a synthetic gene that was resistant to RNA interference (RNAi) due to silent point mutations.The results suggest traffic of RNA molecules from host plants into B.graminis and may lead to an RNAi-based crop protection strategy against fungal pathogens.

英语翻译Powdery mildew fungi are obligate biotrophic pathogens that only grow on living hosts and cause damage in thousands of plant species.Despite their agronomical importance,little direct functional evidence for genes of pathogenicity and vir
白粉病菌是专性植物病毒,只有生长在活宿主,并造成损害的成千上万种植物的.尽管他们的农艺重要性,致病性和毒力的基因几乎没有直接的证据功能目前可以因为诱变和改造协议所缺乏的.这里,我们表明,靶向真菌转录双链或反义RNA在大麦中的积累（大麦）和小麦（普通小麦）影响白粉病菌小麦白粉病的菌发展.匹配的抗性基因Mla10都是由沉默效应子基因Avra10 ,这导致减少的真菌发育在没有获得概念主机诱导的基因沉默的证明,但不是在存在.这种真菌可从Avra10的沉默,这是抵抗RNA干扰（RNAi ）因沉默点突变合成基因的瞬时表达获救.结果表明RNA分子从寄主植物的流量转化为乙菌,并可能导致对真菌病原体的RNAi为基础的作物保护策略.

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