做DNA PCR扩增,最终结果只有阳性对照有条带出来,其它样本都没有出结果,是因为DNA发生了什么变化吗?开始第一次是有阳性条带跟两条样本条带做出来了。
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做DNA PCR扩增,最终结果只有阳性对照有条带出来,其它样本都没有出结果,是因为DNA发生了什么变化吗?开始第一次是有阳性条带跟两条样本条带做出来了。
做DNA PCR扩增,最终结果只有阳性对照有条带出来,其它样本都没有出结果,是因为DNA发生了什么变化吗?
开始第一次是有阳性条带跟两条样本条带做出来了。
做DNA PCR扩增,最终结果只有阳性对照有条带出来,其它样本都没有出结果,是因为DNA发生了什么变化吗?开始第一次是有阳性条带跟两条样本条带做出来了。
这说明你的操作没有问题,只是你的样本确实是阴性的.至于你的样本为什么是阴性,要看你的阳性对照到底是哪里改变了.
If you included positive and negative controls, and if your positive is positive and negative is negative, then whatever the results you obtained is true. The possible reason why your samples become n...
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If you included positive and negative controls, and if your positive is positive and negative is negative, then whatever the results you obtained is true. The possible reason why your samples become negative is as followed:
1. you have DNase contamination with your samples, degradating your samples DNA.
2. You did not included enough template DNA in your second test.
3. check whether you included right primers and whether you included enough.
Good luck!
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第一次和第二次有什么操作不同?
对二楼的Chinglish拜服~~